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A specific and rapid diagnostic tool has been developed to detect Xanthomonas campestris pv. musacearum, the causal agent of bacterial wilt of banana. PCR primers were developed from intergenic regions of X. campestris pv. musacearum following its partial sequence. A total of 48 primers were test...


Adikini, S.Tripathi, L.Beed, Fen D.Tusiime, GeoffreyMagembe, E.M.Kim, D.J.[Development of a specific molecular tool for detecting Xanthomonas campestris pv. musacearum]Development of a specific molecular tool for detecting Xanthomonas campestris pv. musacearum

Investigations were conducted to select the appropriate Agrobacterium tumefaciens strains for efficient transformation of the banana cultivar ‘Williams’. Three A. tumefaciens strains (EHA105, C58, and GV2260) were used for the study. The ability of these strains to transfer β-glucuronidase (GUS) ...


Esuola, C.O.Tripathi, L.Fawole, I.[Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams]Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams

The banana Xanthomonas wilt (BXW) disease, caused by the bacteriumXanthomonas campestris pv. musacearum (XCM), endangers the livelihoods ofmillions of farmers in the Great Lakes region of East and Central Africa. Thedisease was first identified in Uganda in 2001 and has since then also been repor...


Tripathi, L.Tripathi, J.N.Tushemereirwe, W.K.[Control of banana Xanthomonas wilt disease using genetic engineering]Control of banana Xanthomonas wilt disease using genetic engineering

In the more developed countries, genetically engineered crops contributegreatly to agricultural productivity and sustainability. Over the last few years, thelargest growth in the adoption of genetically engineered crops has been in developingcountries and this trend is expected to continue. The m...


Shotkoski, F.A.Tripathi, L.Kiggundu, A.Arinaitwe, G.Tushemereirwe, W.K.[Role of biotechnology and transgenics in bananas (Musa spp.) in Africa]Role of biotechnology and transgenics in bananas (Musa spp.) in Africa

Banana production in Eastern Africa is threatened by the presence of a new devastating bacterial disease caused by Xanthomonas vasicola pv. musacearum (formerly Xanthomonas campestris pv. musacearum). The disease has been identified in Uganda, Eastern Democratic Republic of Congo, Rwanda and Tanz...


Biruma, M.Pillay, M.Tripathi, L.Blomme, GuyAbele, S.Mwangi, M.Bandyopadhyay, RanajitMuchunguzi, P.Kassim, S.Nyine, M.Turyagyenda, L.F.Eden-Green, S.[Banana Xanthomonas wilt: a review of the disease, management strategies and future research directions]Banana Xanthomonas wilt: a review of the disease, management strategies and future research directions

Banana Xanthomonas wilt, caused by Xanthomonas campestris pv. musacearum, is a new threat to banana cultivation in eastern Africa. The causal bacterium grows slowly in culture and is easily overgrown by contaminants. A selective culture medium for isolation of X. c. pv. musacearum will facilitate...


Tripathi, L.Tripathi, J.N.Tushemereirwe, W.K.Bandyopadhyay, Ranajit[Development of a semi selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants]Development of a semi selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants
Autor: Tripathi, L.

The cultivation of genetically modified crops is becoming increasingly important; more traits are emerging and more acres than ever before are being planted with GM varieties. The release of GM crops and products in the markets worldwide has increased the regulatory need to monitor and verify the...


Tripathi, L.[Techniques for detecting genetically modified crops and products]Techniques for detecting genetically modified crops and products

An efficient, simple, and rapid regeneration system was established for bananas (Musa sp.) using sections of corm containing intercalary meristematic tissues as explants. Six different cultivars of banana (‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali ndiizi’, and ‘FHIA-17’) with...


Tripathi, L.Tripathi, J.N.[High frequency shoot regeneration of various cultivars of banana (Musa sp.)]High frequency shoot regeneration of various cultivars of banana (Musa sp.)

The banana Xanthomonas wilt disease (BXW) has threatened the livelihood of millions of farmers in East Africa. Use of resistant varieties is the most cost-effective method of managing this bacterial disease. A reliable and rapid screening method is needed to select resistant banana varieties. An ...


Tripathi, L.Odipio, J.Tripathi, J.N.Tusiime, Geoffrey[A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt]A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt

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