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Enset (Ensete ventricosum), also known as Ethiopian banana, is a food security crop for more than 20 million people in Ethiopia. As conventional breeding of enset is very challenging, genetic engineering is an alternative option to introduce important traits such as enhanced disease resistance an...


Matheka, J.Tripathi, J.N.Merga, I.Gebre, E.Tripathi, L.[A simple and rapid protocol for the genetic transformation of Ensete ventricosum]A simple and rapid protocol for the genetic transformation of Ensete ventricosum

Immature cotyledons collected at different time intervals from four genotypes of chickpea (C 235, BG 256, P 362 and P 372) were cultured adaxially on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine, thidiazuron, kinetin, zeatin and dimethylallylaminopurine (2-iP), either alone o...


Tripathi, L.Singh, A.K.Singh, S.Singh, R.Chaudhary, S.Sanyal, I.Amla, D.[Optimization of regeneration and Agrobacteriummediated transformation of immature cotyledons of chickpea (Cicer arietinum L.)]Optimization of regeneration and Agrobacteriummediated transformation of immature cotyledons of chickpea (Cicer arietinum L.)

Although genetic transformation of clonally propagated crops has been widely studied as a tool for crop improvement and as a vital part of the development of functional genomics resources, there has been no report of any existing Agrobacterium-mediated transformation of yam (Dioscorea spp.) with ...


Nyaboga, E.Tripathi, J.N.Manoharan, R.Tripathi, L.[Agrobacteriummediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement]Agrobacteriummediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement

Banana (Musa spp.) is an important staple food as well as cash crop in tropical and subtropical countries. Various bacterial, fungal, and viral diseases and pests such as nematodes are major constraints in its production and are currently destabilizing the banana production in sub-Saharan Africa....


Tripathi, J.N.Oduor, R.O.Tripathi, L.[A high-throughput regeneration and transformation platform for production of genetically modified banana]A high-throughput regeneration and transformation platform for production of genetically modified banana
Autor: Tripathi, L.

Biotechnology has opened unprecedented avenues for exploring biological systems. Transgenics is one of the key techniques particularly useful for the genetic improvement of crops that are not amenable to conventional breeding, such as those that are vegetatively propagated. In IITA, transgenic te...


Tripathi, L.[Transgenics in crop improvement research at IITA]Transgenics in crop improvement research at IITA

East Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and sl...


Tripathi, L.Tripathi, J.N.Tushemereirwe, W.K.[Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues]Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues

High necrosis and poor survival rate of target plant tissues are some of the major factors that affect the efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors may be the result of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, ...


Kuta, D.Tripathi, L.[Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer]Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer

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