An Agrobacterium-mediated plant transformation system was developed for the production of transgenic plantain [Musa spp. cultivar Agbagba (AAB)]. Apical shoot tips were transformed using Agrobacterium strain EHA105 with the binary vector pCAMBIA 1201, having the hygromycin resistance gene as a se...
Investigations were conducted to select the appropriate Agrobacterium tumefaciens strains for efficient transformation of the banana cultivar ‘Williams’. Three A. tumefaciens strains (EHA105, C58, and GV2260) were used for the study. The ability of these strains to transfer β-glucuronidase (GUS) ...
Esuola, C.O.
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Tripathi, L.
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Fawole, I.
,
[Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams]
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Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams
Bananas and plantains (Musa sp.) are the most important staple food and source of carbohydrates in many countries of Africa. The production is often constrained by many pests and diseases. In order to augment conventional breeding and to avoid constraints imposed by some pests and pathogens, tran...
Banana and plantain (Musa spp.) are major staple foods and a source of income for millions in tropical and subtropical regions. Most of the bananas and plantains grown worldwide are produced by small-scale farmers for home consumption or sale in local and regional markets. Many pests and diseases...
Pseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The iden...
Onyilo, F.
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Tusiime, Geoffrey
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Tripathi, J.N.
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Chen, L.H.
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Falk, B.
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Stergiopoulos, I.
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Tushemereirwe, W.K.
,
Kubiriba, J.
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Tripathi, L.
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[Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants]
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Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants
Atkinson, H.J.
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Roderick, H.
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Tripathi, L.
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[Africa needs streamlined regulation to support the deployment of GM crops]
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Africa needs streamlined regulation to support the deployment of GM crops
Plant parasitic nematodes impose losses of up to 70% on plantains and cooking bananas in Africa.
Application of nematicides is inappropriate and resistant cultivars are unavailable. Where grown, demand
for plantain is more than for other staple crops. Confined field testing demonstrated that tran...
Tripathi, L.
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Babirye, A.
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Roderick, H.
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Tripathi, J.N.
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Changa, C.
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Urwin, P.E.
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Tushemereirwe, W.K.
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Coyne, D.L.
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Atkinson, H.J.
,
[Field resistance of transgenic plantain to nematodes has potential for future African food security]
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Field resistance of transgenic plantain to nematodes has potential for future African food security
In banana and plantain research, it is essential to establish embryogenic cell suspensions together with a highly efficient regeneration and transformation system. This article describes the development of an embryogenic cell suspension (ECS), regeneration, and transformation for plantain cv. “Go...
Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode diges...
Roderick, H.
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Tripathi, L.
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Babirye, A.
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Wang, D.
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Tripathi, J.N.
,
Urwin, P.E.
,
Atkinson, H.J.
,
[Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes]
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Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
Pests and diseases have become major constraints to smallholder banana production in the highlands of East Africa. Biotic challenges to banana cultivation include fungi, bacteria, viruses, nematodes and insects. Host-plant resistance, developed either by conventional breeding or by application of...
Lorenzen, J.H.
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Tenkouano, A.
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Bandyopadhyay, Ranajit
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Vroh Bi, Irie
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Coyne, D.L.
,
Tripathi, L.
,
[The role of crop improvement in pest and disease management]
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The role of crop improvement in pest and disease management