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Dwivedi, S.L.Britt, A.B.Tripathi, L.Sharma, S.Upadhyaya, Hari D.Ortiz, R.[Haploids: constraints and opportunities in plant breeding]Haploids: constraints and opportunities in plant breeding

Banana Xanthomonas wilt (BXW), caused by Xanthomonas campestris pv. musacearum, is one of the most important diseases of banana (Musa sp.) and currently considered as the biggest threat to banana production in Great Lakes region of East and Central Africa. The pathogen is highly contagious and it...


Namukwaya, B.Tripathi, L.Tripathi, J.N.Arinaitwe, G.Mukasa, S.B.Tushemereirwe, W.K.[Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease]Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease

Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomy...


Onyilo, F.Tusiime, GeoffreyChen, L.H.Falk, B.Stergiopoulos, I.Tripathi, J.N.Tushemereirwe, W.K.Kubiriba, J.Changa, C.Tripathi, L.[Agrobacterium tumefaciens-mediated transformation of Pseudocercospora fijiensis to determine the role of PfHog1 in osmotic stress regulation and virulence modulation]Agrobacterium tumefaciens-mediated transformation of Pseudocercospora fijiensis to determine the role of PfHog1 in osmotic stress regulation and virulence modulation

Enset (Ensete ventricosum (Welw.) Cheesman) is one of the Ethiopia’s indigenous sustainability crops supporting the livelihoods of about 20 million people, mainly in the densely populated South and Southwestern parts of the country. Enset serves as a food security crop for humans, animal feed, an...


Merga, I.F.Tripathi, L.Hvoslef-Eide, A.K.Gebre, E.[Application of genetic engineering for control of bacterial wilt disease of enset, Ethiopia's sustainability crop]Application of genetic engineering for control of bacterial wilt disease of enset, Ethiopia's sustainability crop

An Agrobacterium-mediated plant transformation system was developed for the production of transgenic plantain [Musa spp. cultivar Agbagba (AAB)]. Apical shoot tips were transformed using Agrobacterium strain EHA105 with the binary vector pCAMBIA 1201, having the hygromycin resistance gene as a se...


Tripathi, L.Tripathi, J.Hughes, J.[Agrobacterium mediated transformation of plantain (Musa spp.) cultivar Agbagba]Agrobacterium mediated transformation of plantain (Musa spp.) cultivar Agbagba

A specific and rapid diagnostic tool has been developed to detect Xanthomonas campestris pv. musacearum, the causal agent of bacterial wilt of banana. PCR primers were developed from intergenic regions of X. campestris pv. musacearum following its partial sequence. A total of 48 primers were test...


Adikini, S.Tripathi, L.Beed, Fen D.Tusiime, GeoffreyMagembe, E.M.Kim, D.J.[Development of a specific molecular tool for detecting Xanthomonas campestris pv. musacearum]Development of a specific molecular tool for detecting Xanthomonas campestris pv. musacearum

Investigations were conducted to select the appropriate Agrobacterium tumefaciens strains for efficient transformation of the banana cultivar ‘Williams’. Three A. tumefaciens strains (EHA105, C58, and GV2260) were used for the study. The ability of these strains to transfer β-glucuronidase (GUS) ...


Esuola, C.O.Tripathi, L.Fawole, I.[Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams]Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams

The banana Xanthomonas wilt (BXW) disease, caused by the bacteriumXanthomonas campestris pv. musacearum (XCM), endangers the livelihoods ofmillions of farmers in the Great Lakes region of East and Central Africa. Thedisease was first identified in Uganda in 2001 and has since then also been repor...


Tripathi, L.Tripathi, J.N.Tushemereirwe, W.K.[Control of banana Xanthomonas wilt disease using genetic engineering]Control of banana Xanthomonas wilt disease using genetic engineering

In the more developed countries, genetically engineered crops contributegreatly to agricultural productivity and sustainability. Over the last few years, thelargest growth in the adoption of genetically engineered crops has been in developingcountries and this trend is expected to continue. The m...


Shotkoski, F.A.Tripathi, L.Kiggundu, A.Arinaitwe, G.Tushemereirwe, W.K.[Role of biotechnology and transgenics in bananas (Musa spp.) in Africa]Role of biotechnology and transgenics in bananas (Musa spp.) in Africa

Banana production in Eastern Africa is threatened by the presence of a new devastating bacterial disease caused by Xanthomonas vasicola pv. musacearum (formerly Xanthomonas campestris pv. musacearum). The disease has been identified in Uganda, Eastern Democratic Republic of Congo, Rwanda and Tanz...


Biruma, M.Pillay, M.Tripathi, L.Blomme, GuyAbele, S.Mwangi, M.Bandyopadhyay, RanajitMuchunguzi, P.Kassim, S.Nyine, M.Turyagyenda, L.F.Eden-Green, S.[Banana Xanthomonas wilt: a review of the disease, management strategies and future research directions]Banana Xanthomonas wilt: a review of the disease, management strategies and future research directions

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