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In teleostei, as opposed to what happens in mammals, most of the studies that evaluate the quality storages of semen are oriented toward the exposure of spermatozoa to some reactive oxygen species (ROS), the utilization of antioxidants in the diet, or the incorporation of these in seminal plasma....

A diferencia de lo que ocurre en mamíferos, en teleósteos la mayoría de los estudios que evalúan la calidad del semen almacenado están orientados a la exposición de los espermatozoides a algunas especies reactivas de oxígeno (ROS), a la incorporación de antioxidantes en la dieta o a la aplicación...


Berríos, OlgaValdebenito Isler, IvánTreulén, F.Ubilla Madrid, Andrea[Cold storage of sperm of rainbow trout (Oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential][Almacenamiento en frío de espermatozoides de trucha arcoiris (Oncorhynchus mykiss): Efectos en la motilidad, superóxido intracelular, integridad de la membrana plasmática y potencial de membrana mitocondrial]Almacenamiento en frío de espermatozoides de trucha arcoiris (Oncorhynchus mykiss): Efectos en la motilidad, superóxido intracelular, integridad de la membrana plasmática y potencial de membrana mitocondrial

In terms of fish production, sperm activating substances are important in the controlled insemination. This holds also for salmonids, when being provided with an agent that energizes the spermatozoa. In this study two amino acids (l-glutamate and glycine) were evaluated in their effect on sperm m...


Valdebenito Isler, IvánMoreno, C.Lozano, C.Ubilla Madrid, Andrea[Effect of l-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa]Effect of l-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa

In teleostei, as opposed to what happens in mammals, most of the studies that evaluate the quality storages of semen are oriented toward the exposure of spermatozoa to some reactive oxygen species (ROS), the utilization of antioxidants in the diet, or the incorporation of these in seminal plasma....


Berrios, O.Valdebenito Isler, IvánTreulen, F.Ubilla Madrid, Andrea[Cold storage of sperm of rainbow trout (Oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential]Cold storage of sperm of rainbow trout (Oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential

The present investigation determined how different antioxidants incorporated into the sperm diluent for cold storage of semen affected sperm motility and spermatozoan fertility capabilities of the rainbow trout. For the evaluations, fresh semen (C) and semen that had been stored without diluents ...


Ubilla Madrid, AndreaValdebenito Isler, Iván[Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability]Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...


Figueroa Villalobos, ElíasValdebenito Isler, IvánMerino, O.Ubilla Madrid, AndreaRisopatron, J.Farias, J. G.[Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology]Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viabilit...


Ubilla Madrid, AndreaValdebenito Isler, IvánArias, M. E.Risopatron, J.[Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C]Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C

In the following investigation the morphometric characteristics of the first two blastomeres of rainbow trout (Oncorhynchus mykiss) were determined. Embryos were incubated at 9 degrees C and then fixed in a Stockard solution every 30 min starting from 8.5 to 12.5 h of incubation post fertilizatio...


Valdebenito Isler, IvánSanchez, Ruben R.Effer Roldan, BrianUbilla Madrid, Andrea[Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)]Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)

There is a lack of information on the morphology of the first blastomeres that could be used as a diagnostic tool for the first stages of embryonic development for Coho salmon. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of Coho salmon ...


Effer Roldan, BrianSanchez Rueda, RubenUbilla Madrid, AndreaFigueroa Villalobos, EliasValdebenito Isler, Iván[Study of the first blastomeres in Coho salmon (Oncorhynchus kisutch)]Study of the first blastomeres in Coho salmon (Oncorhynchus kisutch)

P>In terms of fish production, sperm activating substances are important in the controlled insemination. This holds also for salmonids, when being provided with an agent that energizes the spermatozoa. In this study two amino acids (l-glutamate and glycine) were evaluated in their effect on sperm...


Valdebenito Isler, IvánMoreno, C.Lozano, C.Ubilla Madrid, Andrea[Effect of L-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa]Effect of L-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa

For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the f...


Effer Roldan, BrianSanchez, Ruben R.Ubilla Madrid, AndreaFigueroa Villalobos, ElíasValdebenito Isler, Iván[Morphometric of blastomeres in Salmo salar]Morphometric of blastomeres in Salmo salar

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