Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...
Figueroa, Elías
,
Lee Estévez, Manuel
,
Valdebenito, Iván
,
Farías, Jorge G.
,
Romero, Jaime
,
[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]
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Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development
Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...
Figueroa, Elías
,
Farías, Jorge G.
,
Lee-Estevez, Manuel
,
Cerqueira, Vinicius
,
Magnotti, Caio
,
Valdebenito, Iván
,
[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]
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Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species
In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...
Durmone, Kelly
,
Valdebenito, Iván
,
Risopatron, Jennie
,
Figueroa, Elías
,
Díaz, Rommy
,
Farias, Jorge
,
[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]
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Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...