Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...
Figueroa, Elías
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Lee Estévez, Manuel
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Valdebenito, Iván
,
Farías, Jorge G.
,
Romero, Jaime
,
[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]
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Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development
Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...
Figueroa, Elías
,
Farías, Jorge G.
,
Lee-Estevez, Manuel
,
Cerqueira, Vinicius
,
Magnotti, Caio
,
Valdebenito, Iván
,
[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]
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Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species
In fish feeding studies, stomach related parameters are mainly measured in order to have biometric relationships. To determine those parameters a direct and straightforward method for measuring real maximum stomach volume in fish based on Archimedes' principle was tested and compared against a tr...
Salgado Ismodes, Andrés
,
Salgado Leu, Ítalo
,
Valdebenito, Iván
,
[A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research]
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A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research
In this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 degrees C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T-1), 50% sea water (488 mO...
Dumorne, Kelly
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Valdebenito, Iván
,
Contreras, Pablo
,
Ulloa Rodríguez, Patricio
,
Risopatrón, Jennie
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Figueroa, Elías
,
Estévez, Manuel Lee
,
Díaz, Rommy
,
Farías, Jorge
,
[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]
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Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...
Durmone, Kelly
,
Valdebenito, Iván
,
Risopatron, Jennie
,
Figueroa, Elías
,
Díaz, Rommy
,
Farias, Jorge
,
[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]
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Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...