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Article

Autor: Figueroa Villalobos, Elías Valdebenito Isler, Iván Merino, O. Ubilla Madrid, Andrea Risopatron, J. Farias, J. G.

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2909

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Merino, O. ,  Ubilla Madrid, Andrea ,  Risopatron, J. ,  Farias, J. G. ,  [Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology] ,  Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
Article

Autor: Risopatron, J. Merino, O. Cheuqueman, C. Figueroa Villalobos, Elías Sanchez, R. Farias, J. G. Valdebenito Isler, Iván

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2944

Repositorio Digital Universidad Católica de Temuco
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Risopatron, J. ,  Merino, O. ,  Cheuqueman, C. ,  Figueroa Villalobos, Elías ,  Sanchez, R. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)] ,  Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Article

Autor: Vega Aguayo, Rolando Pradenas, Miguel Manuel Estrada, Juan Ramirez, Diego Valdebenito Isler, Iván Mardones Lazcano, Alfonso Dantagnan Dantagnan, Patricio Alfaro, Denis Encina Montoya, Francisco Pichara Morales, Cristian

The current trend in world aquaculture is towards the diversification of cultures, mainly native species. The red cusk eel Genypterus chilensis is a native Chilean species of high gastronomic demand and seasonal exploitation that is projected as a candidate for the development of farming technolo...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2979

Repositorio Digital Universidad Católica de Temuco
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Vega Aguayo, Rolando ,  Pradenas, Miguel ,  Manuel Estrada, Juan ,  Ramirez, Diego ,  Valdebenito Isler, Iván ,  Mardones Lazcano, Alfonso ,  Dantagnan Dantagnan, Patricio ,  Alfaro, Denis ,  Encina Montoya, Francisco ,  Pichara Morales, Cristian ,  [Evaluation and comparison of the efficiency of two incubation systems for Genypterus chilensis (Guichenot, 1848) eggs] ,  Evaluation and comparison of the efficiency of two incubation systems for Genypterus chilensis (Guichenot, 1848) eggs
Artículo de Revista

Autor: Figueroa Villalobos, Elías Farias, J.G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I. Oliveira, R.P.S.

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2260

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Figueroa Villalobos, Elías ,  Farias, J.G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I. ,  Oliveira, R.P.S. ,  [Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Artículo de Revista

Autor: Magnotti, C. Figueroa Villalobos, Elías Farias, J.G. Merino, O. Valdebenito Isler, Iván Oliveira, R.P.S. Cerqueira, V.

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L−1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2259

Repositorio Digital Universidad Católica de Temuco
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Magnotti, C. ,  Figueroa Villalobos, Elías ,  Farias, J.G. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Oliveira, R.P.S. ,  Cerqueira, V. ,  [Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions] ,  Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Artículo de Revista

Autor: Lee Estévez, M. Herrera, L. Díaz, R. Beltrán, J. Figueroa Villalobos, Elías Dumorné, K. Ulloa Rodríguez, P. Short, S. Risopatrón, J. Valdebenito Isler, Iván Farías, J.

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2277

Repositorio Digital Universidad Católica de Temuco
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Lee Estévez, M. ,  Herrera, L. ,  Díaz, R. ,  Beltrán, J. ,  Figueroa Villalobos, Elías ,  Dumorné, K. ,  Ulloa Rodríguez, P. ,  Short, S. ,  Risopatrón, J. ,  Valdebenito Isler, Iván ,  Farías, J. ,  [Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility] ,  Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Artículo de Revista

Autor: Díaz, R. Lee Estévez, M. Quiñoñes, J. Dumorné, K. Short, S. Ulloa Rodríguez, P. Valdebenito Isler, Iván Sepúlveda, N. Farías, J.

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2278

Repositorio Digital Universidad Católica de Temuco
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Díaz, R. ,  Lee Estévez, M. ,  Quiñoñes, J. ,  Dumorné, K. ,  Short, S. ,  Ulloa Rodríguez, P. ,  Valdebenito Isler, Iván ,  Sepúlveda, N. ,  Farías, J. ,  [Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation] ,  Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation
Article

Autor: Ubilla Madrid, Andrea Valdebenito Isler, Iván Arias, M. E. Risopatron, J.

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viabilit...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3012

Repositorio Digital Universidad Católica de Temuco
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Ubilla Madrid, Andrea ,  Valdebenito Isler, Iván ,  Arias, M. E. ,  Risopatron, J. ,  [Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C] ,  Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C
Article

Autor: Jara-Seguel, Pedro Valdebenito Isler, Iván Palma-Rojas, Claudio

Galaxias platei is a Chilean endemic fish, with distribution in an extensive geographic area from the central-Southern zone to the Chilean and Argentinean Patagonia. In this work, the somatic nuclear DNA content (2C-value) of Galaxias platei (Galaxiidae) was determined using Feulgen microdensitom...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3014

Repositorio Digital Universidad Católica de Temuco
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Jara-Seguel, Pedro ,  Valdebenito Isler, Iván ,  Palma-Rojas, Claudio ,  [NUCLEAR DNA CONTENT IN GALAXIAS PLATEI (STEINDACHNER, 1898) (TELEOSTEI: OSMERIFORMES: GALAXIIDAE)] ,  NUCLEAR DNA CONTENT IN GALAXIAS PLATEI (STEINDACHNER, 1898) (TELEOSTEI: OSMERIFORMES: GALAXIIDAE)
Article

Autor: Parada Zamorano, Esperanza Peredo, Santiago Cardenas, Silvia Valdebenito Isler, Iván Peredo, Matias

The aim of the present study was to experimentaly evaluate the capability of the freshwater mussel Diplodon chilensis as a potential depurator of residual waters in fish farming. With this purpose, the present study was carried out at the fish farm of the School of Aquaculture of the Catholic Uni...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3037

Repositorio Digital Universidad Católica de Temuco
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Parada Zamorano, Esperanza ,  Peredo, Santiago ,  Cardenas, Silvia ,  Valdebenito Isler, Iván ,  Peredo, Matias ,  [Diplodon chilensis Gray, 1828 (Bivalvia : Hyriidae) a potential residual waters depurator on inland water salmonid, fish-farms: A laboratory scale study] ,  Diplodon chilensis Gray, 1828 (Bivalvia : Hyriidae) a potential residual waters depurator on inland water salmonid, fish-farms: A laboratory scale study

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