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There is a lack of information on the morphology of the first blastomeres that could be used as a diagnostic tool for the first stages of embryonic development for Coho salmon. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of Coho salmon ...


Effer Roldan, BrianSanchez Rueda, RubenUbilla Madrid, AndreaFigueroa Villalobos, EliasValdebenito Isler, Iván[Study of the first blastomeres in Coho salmon (Oncorhynchus kisutch)]Study of the first blastomeres in Coho salmon (Oncorhynchus kisutch)

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...


Merino, O.Risopatron, J.Sanchez, R.Isachenko, E.Figueroa Villalobos, ElíasValdebenito Isler, IvánIsachenko, V.[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness

In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externall...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyFigueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, IvánFarias, Jorge G.[Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)]Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...


Lee Estevez, ManuelHerrera, LisandraDiaz, RommyBeltran, JorgeFigueroa Villalobos, ElíasDumorne, KellyUlloa Rodriguez, PatricioShort, StefaniaRisopatron, JennieValdebenito Isler, IvánFarias, Jorge[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (Delta Psi Mit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into thre...


Bravo, WilliamsDumorne, KellyBeltran Lissabet, JorgeJara Seguel, PedroRomero, JaimeFarias, Jorge G.Risopatron, JennieValdebenito Isler, IvánFigueroa Villalobos, Elías[Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa]Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa

Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...


Magnotti, CaioCerqueira, ViniciusLee Estevez, ManuelFarias, Jorge G.Valdebenito Isler, IvánFigueroa Villalobos, Elías[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species
Figueroa Villalobos, ElíasLee Estevez, M.Risopatron, J.Valdebenito Isler, IvánPinheiro de Souza, R.Farias, J. G.[EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)]EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)

Among all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels ha...


Beltran Lissabet, Jorge FelixHerrera Belen, LisandraLee Estevez, ManuelRisopatron, JennieValdebenito Isler, IvánFigueroa Villalobos, ElíasFarias, Jorge G.[The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)]The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)

The development of sperm cryopreservation has enabled transcendental changes to occur in the reproductive biotechnology of both mammals and fish; it has become a basic tool for animal improvement. Nevertheless, these protocols cause damage to cell structure and physiology, altering sperm function...


Figueroa Villalobos, ElíasValdebenito Isler, IvánZepeda, Andrea B.Figueroa, Carolina A.Dumorne, KellyCastillo, Rodrigo L.Farias, Jorge G.[Effects of cryopreservation on mitochondria of fish spermatozoa]Effects of cryopreservation on mitochondria of fish spermatozoa

There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically...


Valdebenito Isler, IvánFigueroa Villalobos, EliasSandoval Vargas, LeydyContreras Mellado, PabloSanchez Caamano, Juan CarlosFarias Avendano, JorgeRisopatron, Jennie[Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)]Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)

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