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Sperm cryopreservation has led to transcendental changes in the reproductive biotechnology of both mammals and fish, and is a basic tool for animal improvement. However, these protocols generate damage to cell structure and physiology, altering sperm function as a result of cryoinjuries during fr...


Figueroa Villalobos, ElíasValdebenito Isler, IvánFarias, Jorge G.[Technologies used in the study of sperm function in cryopreserved fish spermatozoa]Technologies used in the study of sperm function in cryopreserved fish spermatozoa

Short-term storage of semen is a necessary key procedure in fish; it allows maximizing the use of gametes. Nevertheless, sperm quality decreases during storage has been associated with oxidative stress damage due to an increase in reactive oxygen species (ROS) during storage. This study was desig...


Merino, OsvaldoDumorne, KellySandoval Vargas, LeydyFigueroa Villalobos, ElíasValdebenito Isler, IvánFarias, Jorge G.Risopatron, Jennie[Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function]Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...


Contreras, P.Ulloa, P.Merino, O.Valdebenito Isler, IvánFigueroa Villalobos, ElíasFarias, J.Risopatron, J.[Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm]Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity (vol 533, 736151, 2021)]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity (vol 533, 736151, 2021)

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...


Trigo, P.Merino, O.Figueroa Villalobos, ElíasValdebenito Isler, IvánSanchez, R.Risopatron, J.[Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry]Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
Ulloa, P.Contreras, P.Dumorne, K.Lee-Estevez, M.Figueroa Villalobos, ElíasValdebenito Isler, IvánRisopatron, J.Farias, J. G.[Patagonian blenny (Eleginops maclovinus) spermatozoa characterization and quality markers evaluation under chilling storage.]Patagonian blenny (Eleginops maclovinus) spermatozoa characterization and quality markers evaluation under chilling storage.

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...


Diaz, RommyLee-Estevez, ManuelQuinones, JohnDumorne, KellyShort, StefaniaUlloa-Rodriguez, PatricioValdebenito Isler, IvánSepulveda, NestorFarias, Jorge G.[Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation]Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

'Puye' (Galaxias maculatus) is a small freshwater fish species of great interest to Chilean aquaculture diversification, because of the high commercial value reached by its transparent larvae or 'cristalino'. This study presents the first data with regard to blood cytology in larvae and adults of...


Valdebenito Isler, IvánBusse, K.Jaramillo Brun, NathalieHernández Arias, Adrián [Blood cytology of the common jollytail (Galaxias maculatus) (Jenyns, 1842) (Osmeriformes: Galaxiidae) at postlarval and adult stages]Blood cytology of the common jollytail (Galaxias maculatus) (Jenyns, 1842) (Osmeriformes: Galaxiidae) at postlarval and adult stages

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...


Merino, O.Figueroa Villalobos, ElíasCheuqueman, C.Valdebenito Isler, IvánIsachenko, V.Isachenko, E.Sanchez, R.Farias, J.Risopatron, J.[Short-term storage of salmonids semen in a sodium alginate-based extender]Short-term storage of salmonids semen in a sodium alginate-based extender

Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...


Figueroa Villalobos, ElíasLee Estevez, ManuelValdebenito Isler, IvánFarias, Jorge G.Romero, Jaime[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

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