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Artículo de Revista 2012-02-08

Autor: Merino, O. Risopatrón, J. Sánchez, R. Isachenko, E. Figueroa Villalobos, Elías Valdebenito Isler, Iván Isachenko, V.

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/595

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Risopatrón, J. ,  Sánchez, R. ,  Isachenko, E. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  [Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness] ,  Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Artículo de Revista

Autor: Figueroa Villalobos, Elías Farias, J.G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I. Oliveira, R.P.S.

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2260

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Farias, J.G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I. ,  Oliveira, R.P.S. ,  [Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Artículo de Revista

Autor: Magnotti, C. Figueroa Villalobos, Elías Farias, J.G. Merino, O. Valdebenito Isler, Iván Oliveira, R.P.S. Cerqueira, V.

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L−1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2259

Repositorio Digital Universidad Católica de Temuco
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Magnotti, C. ,  Figueroa Villalobos, Elías ,  Farias, J.G. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Oliveira, R.P.S. ,  Cerqueira, V. ,  [Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions] ,  Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Artículo de Revista

Autor: Lee Estévez, M. Herrera, L. Díaz, R. Beltrán, J. Figueroa Villalobos, Elías Dumorné, K. Ulloa Rodríguez, P. Short, S. Risopatrón, J. Valdebenito Isler, Iván Farías, J.

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2277

Repositorio Digital Universidad Católica de Temuco
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Lee Estévez, M. ,  Herrera, L. ,  Díaz, R. ,  Beltrán, J. ,  Figueroa Villalobos, Elías ,  Dumorné, K. ,  Ulloa Rodríguez, P. ,  Short, S. ,  Risopatrón, J. ,  Valdebenito Isler, Iván ,  Farías, J. ,  [Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility] ,  Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Artículo de Revista

Autor: Díaz, R. Lee Estévez, M. Quiñoñes, J. Dumorné, K. Short, S. Ulloa Rodríguez, P. Valdebenito Isler, Iván Sepúlveda, N. Farías, J.

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2278

Repositorio Digital Universidad Católica de Temuco
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Díaz, R. ,  Lee Estévez, M. ,  Quiñoñes, J. ,  Dumorné, K. ,  Short, S. ,  Ulloa Rodríguez, P. ,  Valdebenito Isler, Iván ,  Sepúlveda, N. ,  Farías, J. ,  [Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation] ,  Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

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