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Artículo de Revista

Autor: Lee Estévez, M. Herrera, L. Díaz, R. Beltrán, J. Figueroa Villalobos, Elías Dumorné, K. Ulloa Rodríguez, P. Short, S. Risopatrón, J. Valdebenito Isler, Iván Farías, J.

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2277

Repositorio Digital Universidad Católica de Temuco
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Lee Estévez, M. ,  Herrera, L. ,  Díaz, R. ,  Beltrán, J. ,  Figueroa Villalobos, Elías ,  Dumorné, K. ,  Ulloa Rodríguez, P. ,  Short, S. ,  Risopatrón, J. ,  Valdebenito Isler, Iván ,  Farías, J. ,  [Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility] ,  Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Artículo de Revista

Autor: Díaz, R. Lee Estévez, M. Quiñoñes, J. Dumorné, K. Short, S. Ulloa Rodríguez, P. Valdebenito Isler, Iván Sepúlveda, N. Farías, J.

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2278

Repositorio Digital Universidad Católica de Temuco
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Díaz, R. ,  Lee Estévez, M. ,  Quiñoñes, J. ,  Dumorné, K. ,  Short, S. ,  Ulloa Rodríguez, P. ,  Valdebenito Isler, Iván ,  Sepúlveda, N. ,  Farías, J. ,  [Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation] ,  Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation
Article

Autor: Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3489

Repositorio Digital Universidad Católica de Temuco
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Lee Estevez, Manuel ,  Herrera, Lisandra ,  Diaz, Rommy ,  Beltran, Jorge ,  Figueroa Villalobos, Elías ,  Dumorne, Kelly ,  Ulloa Rodriguez, Patricio ,  Short, Stefania ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Farias, Jorge ,  [Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility] ,  Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Article

Autor: Beltran Lissabet, Jorge Felix Herrera Belen, Lisandra Lee Estevez, Manuel Risopatron, Jennie Valdebenito Isler, Iván Figueroa Villalobos, Elías Farias, Jorge G.

Among all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels ha...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3535

Repositorio Digital Universidad Católica de Temuco
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Beltran Lissabet, Jorge Felix ,  Herrera Belen, Lisandra ,  Lee Estevez, Manuel ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Figueroa Villalobos, Elías ,  Farias, Jorge G. ,  [The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)] ,  The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)
Article

Autor: Figueroa Villalobos, Elías Farias, J. G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I Oliveira, R. P. S.

The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic ac...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3926

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Farias, J. G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I ,  Oliveira, R. P. S. ,  [Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Article

Autor: Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván

For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the f...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2649

Repositorio Digital Universidad Católica de Temuco
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Effer Roldan, Brian ,  Sanchez, Ruben R. ,  Ubilla Madrid, Andrea ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  [Morphometric of blastomeres in Salmo salar] ,  Morphometric of blastomeres in Salmo salar
Article

Autor: Figueroa Villalobos, Elías Merino, O. Risopatron, J. Isachenko, V. Sanchez, R. Effer Roldan, Brian Isachenko, E. Farias, J. G. Valdebenito Isler, Iván

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3702

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Merino, O. ,  Risopatron, J. ,  Isachenko, V. ,  Sanchez, R. ,  Effer Roldan, Brian ,  Isachenko, E. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification] ,  Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
Article

Autor: Zepeda, Andrea B. Miranda, Ignacia B. Valdebenito Isler, Iván Moreno, Ricardo D. Farias, Jorge G.

The use of synthetic hormones to regulate sexual maturity in captive fish is a common practice. With aquaculture practices, fish production is desired throughout the year, necessitating the maintenance of quality standards, mainly regarding the characteristics of the fish produced. Embryonic deve...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3797

Repositorio Digital Universidad Católica de Temuco
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Zepeda, Andrea B. ,  Miranda, Ignacia B. ,  Valdebenito Isler, Iván ,  Moreno, Ricardo D. ,  Farias, Jorge G. ,  [GnRHa treatments of Atlantic Salmon broodstock suppresses effects of endocrine disruptors, benefitting offspring quality] ,  GnRHa treatments of Atlantic Salmon broodstock suppresses effects of endocrine disruptors, benefitting offspring quality
Article

Autor: Figueroa Villalobos, Elías Lee Estevez, M. Valdebenito Isler, Iván Watanabe, I Oliveira, R. P. S. Romero, J. Castillo, R. L. Farias, J. G.

The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3826

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Watanabe, I ,  Oliveira, R. P. S. ,  Romero, J. ,  Castillo, R. L. ,  Farias, J. G. ,  [Effects of cryopreservation on mitochondrial function and sperm quality in fish] ,  Effects of cryopreservation on mitochondrial function and sperm quality in fish

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