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Article

Autor: Merino, O. Risopatron, J. Sanchez, R. Isachenko, E. Figueroa Villalobos, Elías Valdebenito Isler, Iván Isachenko, V.

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3378

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Risopatron, J. ,  Sanchez, R. ,  Isachenko, E. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  [Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness] ,  Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Article

Autor: Ulloa Rodriguez, Patricio Contreras, Pablo Dumorne, Kelly Figueroa Villalobos, Elías Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge G.

In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externall...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3411

Repositorio Digital Universidad Católica de Temuco
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Ulloa Rodriguez, Patricio ,  Contreras, Pablo ,  Dumorne, Kelly ,  Figueroa Villalobos, Elías ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Farias, Jorge G. ,  [Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)] ,  Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)
Article

Autor: Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3489

Repositorio Digital Universidad Católica de Temuco
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Lee Estevez, Manuel ,  Herrera, Lisandra ,  Diaz, Rommy ,  Beltran, Jorge ,  Figueroa Villalobos, Elías ,  Dumorne, Kelly ,  Ulloa Rodriguez, Patricio ,  Short, Stefania ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Farias, Jorge ,  [Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility] ,  Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Article

Autor: Bravo, Williams Dumorne, Kelly Beltran Lissabet, Jorge Jara Seguel, Pedro Romero, Jaime Farias, Jorge G. Risopatron, Jennie Valdebenito Isler, Iván Figueroa Villalobos, Elías

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (Delta Psi Mit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into thre...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3490

Repositorio Digital Universidad Católica de Temuco
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Bravo, Williams ,  Dumorne, Kelly ,  Beltran Lissabet, Jorge ,  Jara Seguel, Pedro ,  Romero, Jaime ,  Farias, Jorge G. ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Figueroa Villalobos, Elías ,  [Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa] ,  Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa
Article

Autor: Beltran Lissabet, Jorge Felix Herrera Belen, Lisandra Lee Estevez, Manuel Risopatron, Jennie Valdebenito Isler, Iván Figueroa Villalobos, Elías Farias, Jorge G.

Among all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels ha...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3535

Repositorio Digital Universidad Católica de Temuco
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Beltran Lissabet, Jorge Felix ,  Herrera Belen, Lisandra ,  Lee Estevez, Manuel ,  Risopatron, Jennie ,  Valdebenito Isler, Iván ,  Figueroa Villalobos, Elías ,  Farias, Jorge G. ,  [The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)] ,  The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)
Article

Autor: Valdebenito Isler, Iván Figueroa Villalobos, Elias Sandoval Vargas, Leydy Contreras Mellado, Pablo Sanchez Caamano, Juan Carlos Farias Avendano, Jorge Risopatron, Jennie

There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3721

Repositorio Digital Universidad Católica de Temuco
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Valdebenito Isler, Iván ,  Figueroa Villalobos, Elias ,  Sandoval Vargas, Leydy ,  Contreras Mellado, Pablo ,  Sanchez Caamano, Juan Carlos ,  Farias Avendano, Jorge ,  Risopatron, Jennie ,  [Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)] ,  Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)
Article

Autor: Magnotti, C. Figueroa Villalobos, Elías Farias, J. G. Merino, O. Valdebenito Isler, Iván Oliveira, R. P. S. Cerqueira, V.

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3840

Repositorio Digital Universidad Católica de Temuco
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Magnotti, C. ,  Figueroa Villalobos, Elías ,  Farias, J. G. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Oliveira, R. P. S. ,  Cerqueira, V. ,  [Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions] ,  Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Article

Autor: Figueroa Villalobos, Elías Farias, J. G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I Oliveira, R. P. S.

The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic ac...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3926

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Farias, J. G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I ,  Oliveira, R. P. S. ,  [Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Article

Autor: Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván

For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the f...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2649

Repositorio Digital Universidad Católica de Temuco
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Effer Roldan, Brian ,  Sanchez, Ruben R. ,  Ubilla Madrid, Andrea ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  [Morphometric of blastomeres in Salmo salar] ,  Morphometric of blastomeres in Salmo salar
Article

Autor: Peredo, S Parada, E Valdebenito Isler, Iván Peredo, M
Enlace original: http://repositoriodigital.uct.cl/handle/10925/2901

Repositorio Digital Universidad Católica de Temuco
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Peredo, S ,  Parada, E ,  Valdebenito Isler, Iván ,  Peredo, M ,  [Relocation of the freshwater mussel Diplodon chilensis (Hyriidae) as a strategy for its conservation and management] ,  Relocation of the freshwater mussel Diplodon chilensis (Hyriidae) as a strategy for its conservation and management

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