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Studies about nuclear DNA content in the genus Genypterus are scarce, and the present study gives the first estimate of nuclear DNA content of Genypterus chilensis, with a value of 2C= 1.0 +/- 0.06 pg, value close to that obtained for other Ophidiidae species wich range between 1.2 and 1.68 pg.


Jara-Seguel, PedroUbilla Madrid, AndreaEstrada, JuanRamirez, DiegoValdebenito Isler, Iván[Nuclear DNA content in the red conger eel Genypterus chilensis (Guichenot, 1881) (Actinopterygii: Ophidiidae)]Nuclear DNA content in the red conger eel Genypterus chilensis (Guichenot, 1881) (Actinopterygii: Ophidiidae)

Short-term storage of semen is a necessary key procedure in fish; it allows maximizing the use of gametes. Nevertheless, sperm quality decreases during storage has been associated with oxidative stress damage due to an increase in reactive oxygen species (ROS) during storage. This study was desig...


Merino, OsvaldoDumorne, KellySandoval Vargas, LeydyFigueroa Villalobos, ElíasValdebenito Isler, IvánFarias, Jorge G.Risopatron, Jennie[Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function]Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...


Contreras, P.Ulloa, P.Merino, O.Valdebenito Isler, IvánFigueroa Villalobos, ElíasFarias, J.Risopatron, J.[Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm]Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...


Trigo, P.Merino, O.Figueroa Villalobos, ElíasValdebenito Isler, IvánSanchez, R.Risopatron, J.[Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry]Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...


Diaz, RommyLee-Estevez, ManuelQuinones, JohnDumorne, KellyShort, StefaniaUlloa-Rodriguez, PatricioValdebenito Isler, IvánSepulveda, NestorFarias, Jorge G.[Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation]Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

'Puye' (Galaxias maculatus) is a small freshwater fish species of great interest to Chilean aquaculture diversification, because of the high commercial value reached by its transparent larvae or 'cristalino'. This study presents the first data with regard to blood cytology in larvae and adults of...


Valdebenito Isler, IvánBusse, K.Jaramillo Brun, NathalieHernández Arias, Adrián [Blood cytology of the common jollytail (Galaxias maculatus) (Jenyns, 1842) (Osmeriformes: Galaxiidae) at postlarval and adult stages]Blood cytology of the common jollytail (Galaxias maculatus) (Jenyns, 1842) (Osmeriformes: Galaxiidae) at postlarval and adult stages

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...


Merino, O.Figueroa Villalobos, ElíasCheuqueman, C.Valdebenito Isler, IvánIsachenko, V.Isachenko, E.Sanchez, R.Farias, J.Risopatron, J.[Short-term storage of salmonids semen in a sodium alginate-based extender]Short-term storage of salmonids semen in a sodium alginate-based extender

In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...


Dumorne, KellyValdebenito Isler, IvánRisopatron, JennieFigueroa Villalobos, ElíasDiaz, RommyFarias, Jorge[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy
Dumorne, KellyValdebenito Isler, IvánContreras, PabloUlloa Rodriguez, PatricioRisopatron, JennieFigueroa Villalobos, ElíasEstevez, Manuel LeeDiaz, RommyFarias, Jorge[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....


Figueroa Villalobos, ElíasMerino, O.Risopatron, J.Isachenko, V.Sanchez, R.Effer Roldan, BrianIsachenko, E.Farias, J. G.Valdebenito Isler, Iván[Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification]Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

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