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Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...


Contreras, PabloDumorne, KellyUlloa-Rodriguez, PatricioMerino, OsvaldoFigueroa Villalobos, ElíasFarias, Jorge G.Valdebenito Isler, IvánRisopatron, Jennie[Effects of short-term storage on sperm function in fish semen: a review]Effects of short-term storage on sperm function in fish semen: a review

Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...


Dumorne, KellyFigueroa Villalobos, ElíasCosson, JackyLee Estevez, ManuelUlloa Rodriguez, PatricioValdebenito Isler, IvánFarias, Jorge G.[Protein phosphorylation and ions effects on salmonid sperm motility activation]Protein phosphorylation and ions effects on salmonid sperm motility activation

Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...


Magnotti, CaioCerqueira, ViniciusLee Estevez, ManuelFarias, Jorge G.Valdebenito Isler, IvánFigueroa Villalobos, Elías[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

The development of sperm cryopreservation has enabled transcendental changes to occur in the reproductive biotechnology of both mammals and fish; it has become a basic tool for animal improvement. Nevertheless, these protocols cause damage to cell structure and physiology, altering sperm function...


Figueroa Villalobos, ElíasValdebenito Isler, IvánZepeda, Andrea B.Figueroa, Carolina A.Dumorne, KellyCastillo, Rodrigo L.Farias, Jorge G.[Effects of cryopreservation on mitochondria of fish spermatozoa]Effects of cryopreservation on mitochondria of fish spermatozoa

Galaxias maculatus (Jenyns, 1842), is a gourmet fish of great commercial importance with overexploited fisheries. So, studies of its biology are essential in order to develop a technology for its cultivation (galaxiculture), which is at a pilot stage. The goals of this work are: 1) to provide a s...


Vega Aguayo, RolandoDantagnan Dantagnan, PatricioMardones Lazcano, AlfonsoValdebenito Isler, IvánZamorano, JoseEncina Montoya, Francisco[Biological bases for whitebait culture Galaxias maculatus (Jenyns, 1842): a review]Biological bases for whitebait culture Galaxias maculatus (Jenyns, 1842): a review

Many fish species with economic importance have been heavily exploited, causing the collapse of their fisheries. For this reason, there have been attempts to cultivate fish species for commercial purposes, resulting in varying success. It has been observed that when some species are kept in capti...


Valdebenito Isler, IvánPaiva, L.Berland Olea, Marco[Follicular atresia in teleost fish: a review]Follicular atresia in teleost fish: a review

In most teleost fish with external fertilization, the spermatozoids are immobile in the seminal fluid and they are activated only after making contact with all aqueous medium. The time of flagellar movement is short in most species. Furthermore, the factors that trigger this process are different...


Valdebenito Isler, IvánFletcher, C.Vera, V.Fernandez, J.[Physical-chemical factors that regulate spermatic motility in fish: basic and applied aspects. A review]Physical-chemical factors that regulate spermatic motility in fish: basic and applied aspects. A review

The present literature review analyzes the current state of artificial control of sexual maturity in fish culture using hormone therapy, evaluating the advantages and disadvantages of different technological strategies available. The discussion covers from the first successful attempt conducted b...


Valdebenito Isler, Iván[Hormone therapy for the artificial control of sexual maturity in fish culture: a review]Hormone therapy for the artificial control of sexual maturity in fish culture: a review

Sperm cryopreservation has led to transcendental changes in the reproductive biotechnology of both mammals and fish, and is a basic tool for animal improvement. However, these protocols generate damage to cell structure and physiology, altering sperm function as a result of cryoinjuries during fr...


Figueroa Villalobos, ElíasValdebenito Isler, IvánFarias, Jorge G.[Technologies used in the study of sperm function in cryopreserved fish spermatozoa]Technologies used in the study of sperm function in cryopreserved fish spermatozoa

Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...


Figueroa Villalobos, ElíasLee Estevez, ManuelValdebenito Isler, IvánFarias, Jorge G.Romero, Jaime[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

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