Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
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Musoke, A.J.
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Wells, C.W.
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Yagi, Y.
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Nene, Vishvanath
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Spooner, P.R.
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Gachanja, J.
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Osaso, J.
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Bishop, Richard P.
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Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
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Skilton, Robert A.
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Katende, J.
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Wells, C.W.
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Nene, Vishvanath
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McElwain, T.F.
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Morzaria, S.P.
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Musoke, A.J.
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[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
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Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Five adult Boran cattle (Bos indicus), infected with a clone of Trypanosoma congolense IL13-E3 three years earlier and treated, were re-challenged with the same clone. Changes in the peripheral blood were monitored twice weekly, and events in the bone marrow (BM) were assessed by weekly biopsies ...
Anosa, V.O.
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Logan-Henfrey, L.L.
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Wells, C.W.
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[The haematology of Trypanosoma congolense infection in cattle. I. Sequential cytomorphological changes in the blood and bone marrow of Boran cattle]
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The haematology of Trypanosoma congolense infection in cattle. I. Sequential cytomorphological changes in the blood and bone marrow of Boran cattle
Sequential biopsies of sternal bone marrow (BM) of three trypanosusceptible Boran and three trypanotolerant N'Dama cattle were examined by light and transmission electron microscopy before and up to 112 days postinfection (DPI) with Trypanosoma congolense. Before infection, the percentage of cell...
Anosa, V.O.
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Logan-Henfrey, L.L.
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Wells, C.W.
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[The role of the bone marrow in bovine trypanotolerance. II. Macrophage function in Trypanosoma congolense - infected cattle]
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The role of the bone marrow in bovine trypanotolerance. II. Macrophage function in Trypanosoma congolense - infected cattle
Macrophages (Mo) in smears and sections of sternal bone marrow (BM) derived by weekly sequential biopsies from five adult Boran cattle re-challenged with Trypanosoma congolense were studied by light and transmission electron microscopy (TEM). Cells of the mononuclear phagocyte system including mo...
Anosa, V.O.
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Logan-Henfrey, L.L.
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Wells, C.W.
,
[The haematology of Trypanosoma congolense infection in cattle. II. Macrophage structure and function in the bone marrow of Boran cattle]
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The haematology of Trypanosoma congolense infection in cattle. II. Macrophage structure and function in the bone marrow of Boran cattle
This study compared the changes in the bone marrow (BM) of five trypanotolerant N'Dama cattle with those of four trypanosusceptible Boran cattle during trypanosome infection. In the early parasitaemic phase, from 12 to 21 days postinfection (DPI), tsetse transmitted primary Trypanosoma congolense...
Logan-Henfrey, L.L.
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Anosa, V.O.
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Wells, C.W.
,
[The role of the bone marrow in bovine trypanotolerance. 1. Changes in blood and bone marrow in Trypanosoma congolense-infected cattle]
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The role of the bone marrow in bovine trypanotolerance. 1. Changes in blood and bone marrow in Trypanosoma congolense-infected cattle
Bloodstream forms (BSF) and procyclic culture forms (PCF) of African trypanosomes were incubated with a variety of lectins in vitro. Cessation of cell division and profound morphological changes were seen in procyclic forms but not in BSF after incubation with concanvavalin A (Con A). Wheat germ...
Pearson, T.W.
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Beecroft, R.P.
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Welburn, S.C.
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Ruepp, S.
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Roditi, I.
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Kuo-Yuan, H.
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Englund, P.T.
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Wells, C.W.
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Murphy, N.B.
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[The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro]
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The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro
Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...
Skilton, Robert A.
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Musoke, A.J.
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Nene, Vishvanath
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Wasawo, D.P.S.
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Wells, C.W.
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Spooner, P.R.
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Bishop, Richard P.
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Osaso, J.
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Nkonge, C.
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Latif, A.
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Morzaria, S.P.
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[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]
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Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen
Burleigh, B.A.
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Wells, C.W.
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Clarke, M.W.
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Gardiner, Peter R.
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[An integral membrane glycoprotein associated with an endocytic compartment of Trypanosoma vivax. Identification and partial characterization]
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An integral membrane glycoprotein associated with an endocytic compartment of Trypanosoma vivax. Identification and partial characterization
To define Babesia bigemina-specific antigens on the surface of infected erythrocytes, monoclonal antibodies (MAbs) were identified by live-cell immunofluorescence. As determined by live-cell immunofluorescence, two MAbs made to the Mexico strain reacted with the Mexico strain and three Kenya stra...
Shompole, S.
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Perryman, L.E.
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Rurangirwa, F.R.
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McElwain, T.F.
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Jasmer, D.P.
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Musoke, A.J.
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Wells, C.W.
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McGuire, T.C.
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[Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes]
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Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes