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Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...


Tebele, N.Skilton, Robert A.Katende, J.Wells, C.W.Nene, VishvanathMcElwain, T.F.Morzaria, S.P.Musoke, A.J.[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina

To define Babesia bigemina-specific antigens on the surface of infected erythrocytes, monoclonal antibodies (MAbs) were identified by live-cell immunofluorescence. As determined by live-cell immunofluorescence, two MAbs made to the Mexico strain reacted with the Mexico strain and three Kenya stra...


Shompole, S.Perryman, L.E.Rurangirwa, F.R.McElwain, T.F.Jasmer, D.P.Musoke, A.J.Wells, C.W.McGuire, T.C.[Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes]Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes

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