Immunoaffinity chromatography and gel electophoresis were used to isolate a 44 kDa protein that was bound to a 72 kDa chaperone in Trypanosoma brucei brucei. A polyclonal antiserum to the 44 kDa protein was raised in rats and employed in conjunction with chromatography using DEAE-cellulose, Sepha...
Nandam, D.
,
Wells, C.W.
,
Ndegwa, D.
,
Pearson, T.W.
,
[Identification of a 44 kDa protein localized within the endoplasmic reticulum of Trypanosoma brucei brucei]
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Identification of a 44 kDa protein localized within the endoplasmic reticulum of Trypanosoma brucei brucei
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (CB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact CB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 ...
Shompole, S.
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Rurangirwa, F.R.
,
Wambugu, J.
,
Sitienei, J.
,
Mwangi, D.M.
,
Musoke, A.J.
,
Mahan, S.
,
Wells, C.W.
,
McGuire, T.C.
,
[Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains]
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Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains
Grab, D.J.
,
Wells, C.W.
,
Shaw, M.K.
,
Webster, P.
,
Russo, D.C.W.
,
[Endocytosed transferrin in African trypanosomes is delivered to lysomes and may not be recycled]
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Endocytosed transferrin in African trypanosomes is delivered to lysomes and may not be recycled
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
,
Ole-MoiYoi, O.K.
,
Urakawa, T.
,
Hirumi, H.
,
Majiwa, P.A.O.
,
Wells, C.W.
,
Minja, S.H.
,
Makau, J.M.
,
Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
,
Bishop, Richard P.
,
Wells, C.W.
,
Spooner, P.R.
,
Gobright, E.I.
,
Nkonge, C.
,
Musoke, A.J.
,
Macklin, M.D.
,
Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)
Characterising the drug susceptibility of trypanosomes that are pathogenic for domestic livestock has for many years been carried out either in vivo, using large or small animals, or by using various in vitro cultivation methodologies (Peregrine, 1994). Thus, adaptation to, and multiplication of ...
Peregrine, A.S.
,
Wells, C.W.
,
Wilkes, J.M.
,
[Methods for detection of drug resistance in trypanosomes - new developments?]
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Methods for detection of drug resistance in trypanosomes - new developments?
Theileria parva, an obligate intracellular protozoan parasite of cattle, causes East Coast fever, a fatal, lymphoproliferative disease, endemic in large areas of East and Central Africa. The mammalian-infective sporozoites of T. parva mature within the salivary glands of the ixodid tick Rhipiceph...
Wells, C.W.
,
Awino, Elias
,
McKeever, Declan J.
,
[Theileria parva sporozoite invasion and development within bovine dendritic cells]
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Theileria parva sporozoite invasion and development within bovine dendritic cells
Sequential changes in the blood and bone marrow (BM) were studied by light and transmission electron microscopy in three Boran and three Ndama cattle before and after a primary infection with Trypanosoma congolense IL 1180. Parasitaemia was essentially similar in the Borans and the Ndamas between...
Anosa, V.O.
,
Logan-Henfrey, L.L.
,
Wells, C.W.
,
[Bone marrow and macrophage functions as determinants of bovine trypanotolerance]
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Bone marrow and macrophage functions as determinants of bovine trypanotolerance