Immunoaffinity chromatography and gel electophoresis were used to isolate a 44 kDa protein that was bound to a 72 kDa chaperone in Trypanosoma brucei brucei. A polyclonal antiserum to the 44 kDa protein was raised in rats and employed in conjunction with chromatography using DEAE-cellulose, Sepha...
Nandam, D.
,
Wells, C.W.
,
Ndegwa, D.
,
Pearson, T.W.
,
[Identification of a 44 kDa protein localized within the endoplasmic reticulum of Trypanosoma brucei brucei]
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Identification of a 44 kDa protein localized within the endoplasmic reticulum of Trypanosoma brucei brucei
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (CB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact CB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 ...
Shompole, S.
,
Rurangirwa, F.R.
,
Wambugu, J.
,
Sitienei, J.
,
Mwangi, D.M.
,
Musoke, A.J.
,
Mahan, S.
,
Wells, C.W.
,
McGuire, T.C.
,
[Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains]
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Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains
Grab, D.J.
,
Wells, C.W.
,
Shaw, M.K.
,
Webster, P.
,
Russo, D.C.W.
,
[Endocytosed transferrin in African trypanosomes is delivered to lysomes and may not be recycled]
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Endocytosed transferrin in African trypanosomes is delivered to lysomes and may not be recycled
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
,
Ole-MoiYoi, O.K.
,
Urakawa, T.
,
Hirumi, H.
,
Majiwa, P.A.O.
,
Wells, C.W.
,
Minja, S.H.
,
Makau, J.M.
,
Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
,
Bishop, Richard P.
,
Wells, C.W.
,
Spooner, P.R.
,
Gobright, E.I.
,
Nkonge, C.
,
Musoke, A.J.
,
Macklin, M.D.
,
Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)