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African oil palm ringspot virus (AOPRV) had been previously described as a fovea-like virus associated with a lethal disease of African oil palm (Elaeis guineensis) in South America. The original report was based on partial sequence and a distant relationship between AOPRV and Apple stem pitting ...


Lozano Potes, IMorales, Francisco JoséMartínez, A.K.Pena, E[Molecular characterization and detection of African oil palm ringspot virus]Molecular characterization and detection of African oil palm ringspot virus

The complete nucleotide sequences of RNAs 1 and 2 of Rice stripe necrosis virus (RSNV) were determined and compared to the corresponding genomes of all sequenced, rod-shaped plant viruses. The genome organisation of RSNV RNA1 and RNA2 is nearly identical to that of Beet necrotic yellow vein virus...


Lozano Potes, IMorales, Francisco José[Molecular characterisation of rice stripe necrosis virus as a new species of the genus benyvirus]Molecular characterisation of rice stripe necrosis virus as a new species of the genus benyvirus

Systemin is a plant signal peptide hormone involved in the responses to wounding and insect damage in the Solanaceae family. It works in the same signaling pathway of jasmonic acid (JA) and enhances the expression of proteinase inhibitors. With the aim of studying a role for systemin in plant ant...


Bubici, G.Carluccio, A.V.Stavolone, L.Cillo, F.[Prosystemin overexpression induces transcriptional modifications of defense-related and receptor-like kinase genes and reduces the susceptibility to Cucumber mosaic virus and its satellite RNAs in transgenic tomato plants]Prosystemin overexpression induces transcriptional modifications of defense-related and receptor-like kinase genes and reduces the susceptibility to Cucumber mosaic virus and its satellite RNAs in transgenic tomato plants

We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yield...


Behnam, BabakBohórquez Cháux, AdrianaCastañeda-Méndez, Oscar F.Tsuji, HiroyukiIshitani, ManabuBecerra López Lavelle, Luis Augusto[An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)]An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)

For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...


Narina, S.S.Mohammed, I.Asiedu, RobertMignouna, H.D.[A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction]A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

The brown pod-sucking bug, Clavigralla tomentosicollis Stäl (Hemiptera: Coreidae), causes significant damage to cultivated cowpea, Vigna unguiculata Walp, a staple crop in sub-Saharan Africa. C. tomentosicollis pierce and suck sap from cowpea pods, resulting in reduced grain yield and quality. Th...


Steele, L.D.Sun, W.Valero, M.C.Ojo, J.A.Seong, K.M.Coates, B.S.Margam, V.M.Tamò, M.Pittendrigh, Barry R.[The mitogenome of the brown pod-sucking bug Clavigralla tomentosicollis Stäl (Hemiptera: Coreidae)]The mitogenome of the brown pod-sucking bug Clavigralla tomentosicollis Stäl (Hemiptera: Coreidae)

Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived small interfering RNAs has proven to be a highly efficient approach for virus discovery. Here we present VirusDetect, a bioinformatics pipelin...


Yi ZhengShan GaoPadmanabhan, C.Li, R.Galvez, M.Gutiérrez, D.Fuentes, S.Kai-Shu LingKreuze, J.F.Zhangjun Fei[VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.]VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.

Sweet potato [Ipomoea batatas (L.) Lam] is increasingly becoming an important food and industrial crop worldwide that is affected by over thirty viruses belonging to different virus families. Little is known about these viruses on the many local and commercial sweet potato cultivars in the Caribb...


Alleyne, A.T.Cummins, C.Rowe, K.James, M.Fuentes, S.Gutiérrez, D.[Sequencing and assembly of small RNAs reveal the presence of several begomoviruses, potyviruses, badnaviruses and mastreviruses in the sweet potato leaf virome in Barbados.]Sequencing and assembly of small RNAs reveal the presence of several begomoviruses, potyviruses, badnaviruses and mastreviruses in the sweet potato leaf virome in Barbados.
Pelle, RogerMurphy, N.B.[Stage-specific differential polyadenylation of mini-exon derived RNA in African trypanosomes]Stage-specific differential polyadenylation of mini-exon derived RNA in African trypanosomes
Read, L.K.Jacob, N.K.Fish, W.R.Muthiani, A.M.Stuart, K.[Sequences of three Trypanosoma congolense maxicircle genes allow prediction of regions encoding transcripts that undergo extensive RNA editing]Sequences of three Trypanosoma congolense maxicircle genes allow prediction of regions encoding transcripts that undergo extensive RNA editing

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