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The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....


Bishop, Richard P.Geysen, D.Spooner, P.R.Skilton, Robert A.Nene, VishvanathDolan, T.T.Morzaria, S.P.[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle

Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...


Graham, S.P.Saya, R.Awino, EliasNgugi, D.Nyanjui, J.K.Hecker, R.Taracha, E.L.N.Nene, Vishvanath[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva

Theileria parva is an intracellular sporozoan parasite that infects and transforms bovine lymphocytes, causing a severe lymphoproliferative disease known as East Coast fever in eastern, central and southern Africa. In this article, Declan McKeever and colleagues summarize the current understandin...


McKeever, Declan J.Taracha, E.L.N.Morrison, W. IvanMusoke, A.J.Morzaria, S.P.[Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies]Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies

A first-gneration subunit vaccine for East Coast fever based on the Theileria parva p67 sporozoite surface antigen is entering preliminary field trials. Intensive efforts are also focused on the identification of a schizont-specific omponen for incorporation in a second-generation multi-component...


McKeever, Declan J.Morrison, W. Ivan[Invited review Novel vaccines against Theileria parva: prospects for sustainability]Invited review Novel vaccines against Theileria parva: prospects for sustainability

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...


Ballingall, K.T.Mwangi, D.M.MacHugh, Niall D.Taracha, E.L.N.Totte, P.McKeever, Declan J.[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
Shaw, M.K.Tilney, L.G.[How individual cells develop from a syncytium: Merogony in Theileria parva (Apicomplexa)]How individual cells develop from a syncytium: Merogony in Theileria parva (Apicomplexa)

The major histocompatibility complex (MHC) class I molecules are ubiquious cell surface molecules involved in the cell-mediated immune response. We show here, using a number of different, independent approaches, that these proteins are an essential component of the host cell surface receptor invo...


Shaw, M.K.Tilney, L.G.Musoke, A.J.Teale, A.J.[MHC class I molecules are an essential cell surface component involved in Theileria parva sporozoite binding to bovine lymphocytes]MHC class I molecules are an essential cell surface component involved in Theileria parva sporozoite binding to bovine lymphocytes

Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...


Bishop, Richard P.Musoke, A.J.Morzaria, S.P.Sohanpal, B.K.Gobright, E.I.[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...


Kaba, S.A.Musoke, A.J.Schaap, D.Schetters, T.Rowlands, G.J.Vermeulen, A.N.Nene, VishvanathVlak, J.M.Oers, M.M. van[Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle]Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

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