Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...
Nene, Vishvanath
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Inumaru, S.
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McKeever, Declan J.
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Morzaria, S.P.
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Shaw, M.
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Musoke, A.J.
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[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]
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Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle
Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...
Ballingall, K.T.
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Luyai, A.
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Rowlands, G.J.
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Sales, J.
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Musoke, A.J.
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Morzaria, S.P.
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McKeever, Declan J.
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[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]
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Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen
To define Babesia bigemina-specific antigens on the surface of infected erythrocytes, monoclonal antibodies (MAbs) were identified by live-cell immunofluorescence. As determined by live-cell immunofluorescence, two MAbs made to the Mexico strain reacted with the Mexico strain and three Kenya stra...
Shompole, S.
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Perryman, L.E.
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Rurangirwa, F.R.
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McElwain, T.F.
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Jasmer, D.P.
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Musoke, A.J.
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Wells, C.W.
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McGuire, T.C.
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[Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes]
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Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes
The p67 sporozoite antigen of Theileria parva has been fused to the C-terminal secretion signal of Escherichia coli hemolysin and expressed in secreted form by attenuated Salmonella dublin areA strain SL5631. The recombinant p67 antigen was detected in the supernatant of transformed bacterial cul...
Gentschev, I.
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Glaser, I.
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Goebel, W.
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McKeever, Declan J.
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Musoke, A.J.
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Heussler, V.T.
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[Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle]
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Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle
Shapiro, S.Z.
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Kimmel, E.
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[A simple method for the production of specific antiserum to protein encoded in cloned genes. Immunization with precipitin lines]
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A simple method for the production of specific antiserum to protein encoded in cloned genes. Immunization with precipitin lines
Shapiro, S.Z.
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Buscher, G.
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Dobbelaere, D.A.E.
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[Acquired resistance to Rhipicephalus appendiculatus (Acari:Ixodidae). Identification of an antigen eliciting resistance in rabbits]
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Acquired resistance to Rhipicephalus appendiculatus (Acari:Ixodidae). Identification of an antigen eliciting resistance in rabbits
Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...
Totte, P.
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Nyanjui, J.
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Bensaid, A.
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McKeever, Declan J.
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[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]
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Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium
International Laboratory for Research on Animal Diseases
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[ILRAD Reports. Volume 10. Number 3. July 1992]
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ILRAD Reports. Volume 10. Number 3. July 1992
Cattle that recover from Theileria parva infection are solidly immune to rechallenge with homologous parasites. Most of the ILRAD/ILRI immunological research into the disease has focused on defining the basis of this protection. Over 15 years of basic and applied immunological research at ILRAD/I...