The effects of holding temperature, pH and medium on the infectivity of Theileria parva sporozoites were investigated using an in vitro infectivity assay. The sporozoite infectivity lasted for 72 h at a holding temperature of 4 C but for only 24 h at 24 degrees C. Sporozoite infectivity was fo...
Kimbita, E.N.
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Silayo, R.S.
,
Dolan, T.T.
,
[Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity]
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Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity
Among the blood-sucking arthropods, ixodid ticks have some of the most complicated feeding behavior requirements. Feeding is preceded by an attachment process, and each instar has to remain fully attached to the same feeding site for a period of 2-14 days. These biological demands have for a long...
Young, A.S.
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Waladde, S.M.
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Morzaria, S.P.
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[Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission]
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Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission
Ole-MoiYoi, O.K.
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Nayar, A.
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Iams, K.P.
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Musoke, A.J.
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Yilma, T.
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[Molecular aspects of Theileria parva and approaches to vaccine development for animals]
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Molecular aspects of Theileria parva and approaches to vaccine development for animals
The role of calcium in Theileria parva sporozoite entry into bovine lymphocytes was examined. Depletion of Ca2+ from the external medium had little effect on sporozoite entry suggesting that the initial sporozoite-host cell interaction is a Ca2+-independent process. Sporozoite entry could, howeve...
Shaw, M.K.
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[Mobilization of intrasporozoite Ca(2+) is essential for Theileria parva sporozoite invasion of bovine lymphocytes]
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Mobilization of intrasporozoite Ca(2+) is essential for Theileria parva sporozoite invasion of bovine lymphocytes
Kurtti, T.J.
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Munderloh, U.G.
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Irvin, A.D.
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Buscher, G.
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[Theileria parva: Early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts]
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Theileria parva: Early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts
Theileria parva (Boleni) stock from Zimbabwe was used to immunise 24 susceptible Friesian calves by infection and treatment with oxytetracycline. Twenty-eight days after immunisation the animals in groups of 6 plus 2 susceptible controls were subjected to challenge: 3 groups with heterologous sto...
Hove, T.
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Musisi, F.L.
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Kanhai, G.K.
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Latif, A.
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Masaka, S.
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Munatswa, F.C.
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Pegram, R.G.
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Kamwendo, S.P.
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Quiroga, J.C.
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Mwangondwe, R.
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Dolan, T.T.
,
[Challenge of Theileria parva (Boleni) immunised cattle with selected East African Theileria stocks]
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Challenge of Theileria parva (Boleni) immunised cattle with selected East African Theileria stocks
Rhipicephalus appendiculatus adult ticks, collected int he field from zebu (Bos indicus) and exotic (Bos tarus) cattle with high antibody titres to Theileria parva schizont antigen, transmitted Theileria parva infection typical of East Coast fever to susceptible cattle. Uninfected R. appendiculat...
Kariuki, D.P.
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Young, A.S.
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Morzaria, S.P.
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Lesan, A.C.
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Mining, S.K.
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Omwoyo, P.
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Wafula, J.L.M.
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Molyneux, D.H.
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[Theileria parva carrier state in naturally infected and artificially immunized cattle]
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Theileria parva carrier state in naturally infected and artificially immunized cattle
Morrison, W. Ivan
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Goddeeris, M.
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Teale, A.J.
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[Bovine cytotoxic T cell clones which recognise lymphoblasts infected with two antigenically different stocks of the protozoan parasite Theileria parva]
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Bovine cytotoxic T cell clones which recognise lymphoblasts infected with two antigenically different stocks of the protozoan parasite Theileria parva
Nyormoi, O.
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Bwayo, J.J.
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Hirumi, H.
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[Theileria parva: Isolation of macroschizonts from in vitro propagated lymphoblastoid cells of cattle]
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Theileria parva: Isolation of macroschizonts from in vitro propagated lymphoblastoid cells of cattle