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Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...


Ballingall, K.T.Luyai, A.Rowlands, G.J.Sales, J.Musoke, A.J.Morzaria, S.P.McKeever, Declan J.[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen

The p67 sporozoite antigen of Theileria parva has been fused to the C-terminal secretion signal of Escherichia coli hemolysin and expressed in secreted form by attenuated Salmonella dublin areA strain SL5631. The recombinant p67 antigen was detected in the supernatant of transformed bacterial cul...


Gentschev, I.Glaser, I.Goebel, W.McKeever, Declan J.Musoke, A.J.Heussler, V.T.[Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle]Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle
Emery, D.L.Morrison, W. IvanBuscher, G.Nelson, R.T.[Generation of cell-mediated cytotoxicity to Theileria parva (East Coast fever) after inoculation of cattle with parasitized lymphoblasts]Generation of cell-mediated cytotoxicity to Theileria parva (East Coast fever) after inoculation of cattle with parasitized lymphoblasts
Perry, Brian D.Young, A.S.[The naming game. The changing fortunes of East Coast fever and Theileria parva]The naming game. The changing fortunes of East Coast fever and Theileria parva

International Laboratory for Research on Animal Diseases[ILRAD Reports. Volume 10. Number 3. July 1992]ILRAD Reports. Volume 10. Number 3. July 1992

Cattle that recover from Theileria parva infection are solidly immune to rechallenge with homologous parasites. Most of the ILRAD/ILRI immunological research into the disease has focused on defining the basis of this protection. Over 15 years of basic and applied immunological research at ILRAD/I...


McKeever, Declan J.[Multi-component subunit vaccines against Theileria parva]Multi-component subunit vaccines against Theileria parva

The preparation of Theileria parva stabilates has been described by Cunningham et al (1973). The method is detailed in the FAO report (1978) and the FAO tick and tick-borne Disease Manual (1984). Theileria parva stabilates are a source of viable sporozoites that can be used to immunise cattle by ...


Morzaria, S.P.Spooner, P.R.Bishop, Richard P.Mwaura, S.[The preparation of a composite stabilate for immunisation against East Coast fever]The preparation of a composite stabilate for immunisation against East Coast fever

T cells bearing the gamma-delta antigen can constitute up to 50% of T cells in peripheral blood and lymphoid organs of calves below six months of age. This observation prompted us to investigate the involvement of gamma-delta T cells in the immune response of yound calves against Theileria parva.


Daubenberger, C.A.[A role for gamma-delta T cells in theileriosis]A role for gamma-delta T cells in theileriosis

We evaluated sexual recombination in the apicomplexan parasite Theileria parva using genome-wide marker analysis of haploid sporozoite populations obtained from infected Rhipicephalus appendiculatus ticks. Analysis of 231 parasite clones derived by in vitro infection of bovine lymphocytes reveale...


Katzer, F.Ngugi, D.Oura, C.Bishop, Richard P.Taracha, E.L.N.Walker, A.R.McKeever, Declan J.[Extensive genotypic diversity in a recombining population of the apicomplexan parasite Theileria parva]Extensive genotypic diversity in a recombining population of the apicomplexan parasite Theileria parva

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