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A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoame...
Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguic...
We determined DNA fingerprints and pathotypes on international differentials for 151 isolates of the rice blast fungus Pyricularia grisea that were collected from 15 rice cultivars at a blast disease breeding nursery in Santa Rosa, Colombia. The pathogen population expressed an extraordinarily hi...
Bean golden mosaic geminivirus (BGMV) causes major losses on beans (Phaseolus vulgaris) in the tropical and subtropical Americas and the Caribbean Basin. Infectious DNA-A and -B components of BGMV isolates from the Dominican Republic (BGMV-DR) and Guatemala (BGMV-GA) were sequenced, and their gen...
Geminiviruses are small plant viruses that encapsidate a single-stranded circular DNA and form double-stranded DNAs in infected plants. Infection of economically important hosts with cloned DNAs of geminiviruses frequently has been difficult. Four isolates of bipartite bean-infecting geminiviruse...
Breeding of Phaseolus vulgaris L. for resistance to common bacterial blight (CBB) can be done with visual evaluations of symptoms to distinguish broad resistance classes, but a more quantitative measure was needed for genetic studies of resistance. A novel method of evaluation was developed by qu...
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