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Segovia, RJBedoya, ATriviño, WilliamCeballos, H.Gálvez E., Guillermo E.Ospina Patiño, Bernardo[Metodología para el endurecimiento masivo de `vitroplantas` de yuca]Metodología para el endurecimiento masivo de `vitroplantas` de yuca

Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nucl...


Gabriel, C.J.WALSH, R.Nolt, Barry L.[Evidence for a latent viruslike agent in cassava]Evidence for a latent viruslike agent in cassava

Segovia, Roberto J.Bedoya, ArmandoTriviño, WilliamCeballos, H.Fregene, Martin A.Gálvez E., Guillermo E.Ospina, Bernardo[Methodology for hardening large numbers of In vitro cassava plants]Methodology for hardening large numbers of In vitro cassava plants

In 1985, the activities of the Biotechnology Research Unit (CIAT) involved research in cell and tissue culture for clonal propagation and for the generation of useful variability. These activities included work carried out at CIAT and at collaborating institutions abroad. Work at CIAT concentrate...

En 1985, las actividades de la Unidad de Investigacion en Biotecnologia (CIAT) incluyeron la investigacion sobre el cultivo de celulas y de tejidos para la propagacion clonal y para la generacion de variabilidad util. Estas actividades incluyeron trabajos realizados en CIAT y en las instituciones...


International Center for Tropical Agriculture[Actividades de germoplama de frijol. Unidad de Biotecnología]Actividades de germoplama de frijol. Unidad de Biotecnología

During 1986, activities with Phaseolus in the biotechnology research unit focused on: developing tissue culture techniques for regeneration of bean plants using various organs and tissues; developing electrophoretic techniques for genotype identification; and monitoring research activities in asp...

Durante 1986 las actividades con Phaseolus en la unidad de investigaciones en biotecnologia se enfocaron hacia: el desarrollo de tecnicas de cultivo de tejidos para la regeneracion de plantas de frijol utilizando distintos organos y tejidos; el desarrollo de tecnicas de electroforesis para la ide...


International Center for Tropical Agriculture[Actividades con germoplasma de frijol. Variabilidad genética a partir de la biotecnología]Actividades con germoplasma de frijol. Variabilidad genética a partir de la biotecnología

During 1986, activities with Phaseolus in the biotechnology research unit focused on: developing tissue culture techniques for regeneration of bean plants using various organs and tissues; developing electrophoretic techniques for genotype identification; and monitoring research activities in asp...

Durante 1986 las actividades con Phaseolus en la unidad de investigaciones en biotecnologia se enfocaron hacia: el desarrollo de tecnicas de cultivo de tejidos para la regeneracion de plantas de frijol utilizando distintos organos y tejidos; el desarrollo de tecnicas de electroforesis para la ide...


International Center for Tropical Agriculture[Bean germplasm activities. Genetic variability from biotechnological techniques]Bean germplasm activities. Genetic variability from biotechnological techniques

This 14-minute video describes the protocol used for the collection of female flowers at different ages after anthesis and the development of a protocol of early embryo rescue. The production of doubled haploids through different gynogenesis approaches usually require an early rescue of embryos w...


Buitrago, María EugeniaTabares, EddieLentini, ZaidaVanegas, FreddyWedzony, MariaCeballos, Hernán[Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca]Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca

This 9-minute video describes the protocol used for the collection of male flowers in cassava for the production of doubled haploids through androgenesis (in vitro microspore or anther culture). Pre-treatments and different culture conditions used in the process to induce cell division in immatur...


Perera, Prasanthi I.P.Golemiec, ElzbietaDedicova, BeataWedzony, MariaHeberle-Bors, ErwinOrdoñez, CarlosCeballos, Hernán[Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca]Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca

During 1981, the efforts to use meristem-culture methods have continued, together with thermotherapy for recovering healthy clones. Frog skin disease was eradicated up to 100 percent in different cassava clones during various propagation cycles through the culture of small meristem tips. A routin...


International Center for Tropical Agriculture[Cultivo de tejidos]Cultivo de tejidos

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