The membrane-associated form of the variable surface glycoprotein (mfVSG) from African trypanosomes is a potent macrophage activator capable of inducing production of tumor necrosis factor z (TNFalfa) in both bovine and murine models. Using a bovine model. we have re-investigated the hypothesis t...
Sileghem, M.R.
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Saya, R.
,
Grab, D.J.
,
Naessens, Jan
,
[An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes]
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An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes
Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recentl...
Totte, P.
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Bensaid, A.
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Mahan, S.M.
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Martínez, D.
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McKeever, Declan J.
,
[Immune responses to Cowdria ruminantium infections]
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Immune responses to Cowdria ruminantium infections
Bovine bone marrow-derived macrophages were infected in vitro with non-cytopathic or cytopathic strains of bovine viral diarrhoea virus. Infection with both biotypes resulted in a decreased production of tumour necrosis factor alpha upon stimulation with heat-inactivated Salmonella dublin or lipo...
Adler, H.
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Jungi, T.W.
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Pfister, H.
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Strasser, M.
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Sileghem, M.R.
,
Peterhans, E.
,
[Cytokine regulation by virus infection: Bovine viral diarrhea virus, a flavivirus, downregulates production of tumor necrosis factor alpha in macrophages in vitro]
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Cytokine regulation by virus infection: Bovine viral diarrhea virus, a flavivirus, downregulates production of tumor necrosis factor alpha in macrophages in vitro
The TNF-alfa gene on mouse chromosome MMU17 is among the candidates for the trypanosomosis resistance QTG Tirl. Tit t has the largest effect of those loci so far detected which influence degree of resistance to murine trypanosomosis caused by Trypanosome congolense infection. We therefore studied...
Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with try...
Lutje, V.
,
Taylor, K.A.
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Boulange, A.F.
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Authié, E.
,
[Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle]
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Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle
In the present study, we used newly developed F11 generation mouse advanced intercross lines (AIL) to fine map Pas1–3 quantitative trait loci (QTL). The (A/J x C57BL/6) F11 AIL mouse population was created by crossing lung tumor-resistant C57BL/6 mice with lung tumor-susceptible A/J mice. By se...
Wang, M.
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Lemon, W.J.
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Liu, G.
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Wang, Y.
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Iraqi, F.A.
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Malkinson, A.M.
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You, M.
,
[Fine mapping and identification of candidate pulmonary adenoma susceptibility 1 genes using advanced intercross lines]
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Fine mapping and identification of candidate pulmonary adenoma susceptibility 1 genes using advanced intercross lines
In this paper I argue for building dialogues between scientific and Maasai knowledge articulations, utilizing knowledge of wildebeest as an example. By locating Maasai and scientific knowledges regarding a particular subject (wildebeest) in relation to each other-in discourse and in practice-my i...
Goldman, M.
,
[Tracking wildebeest, locating knowledge: Maasai and conservation biology understandings of wildebeest behavior in northern Tanzania]
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Tracking wildebeest, locating knowledge: Maasai and conservation biology understandings of wildebeest behavior in northern Tanzania
We have previously obtained strong evidence for linkage of mild malaria attack to the MHC region, with a peak close to the tumor necrosis factor (TNF) gene. We screened, for polymorphisms, the entire TNF gene in the same sample of 34 families comprising 197 individuals living in a Plasmodium falc...
Flori, L.
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Delahaye, N.F.
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Iraqi, F.A.
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Hernández Valladares, M.
,
Fumoux, F.
,
Rihet, P.
,
[TNF as a malaria candidate gene: Polymorphism-screening and family-based association analysis of mild malaria attack and parasitemia in Burkina Faso]
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TNF as a malaria candidate gene: Polymorphism-screening and family-based association analysis of mild malaria attack and parasitemia in Burkina Faso
Experimental infections of mice with the African trypanosome Trypanosoma brucei lead to a profound state of T-cell unresponsiveness in the lymph node cell (LNC) compartment. This suppression is mediated by macrophage-like cells which inhibit interleukin 2 (IL-2) secretion and down-regulate IL-2 r...
Darji, A.
,
Beschin, A.
,
Sileghem, M.R.
,
Heremans, H.
,
Brys, L.
,
Baetselier, P. de
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[In vitro simulation of immuno-suppression caused by Trypanosoma brucei: Active involvement of gamma interferon and tumor necrosis factor in the pathway of suppression]
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In vitro simulation of immuno-suppression caused by Trypanosoma brucei: Active involvement of gamma interferon and tumor necrosis factor in the pathway of suppression
Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...
Totte, P.
,
Nyanjui, J.
,
Bensaid, A.
,
McKeever, Declan J.
,
[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]
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Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium