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A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced ...
These guidelines are divided into 2 parts. The first makes general recommendations on how best to move Dioscorea germplasm and mentions available intermediate quarantine facilities. The second part covers the important insect and nematode pests and viral and fungal diseases of quarantine concern....
Reliable characterization of the variation among wild and cultivated yams in Nigeria is essential for improved management and efficient utilization of yam genetic resources. RAPD and double stringency PCR (DS-PCR) analyses were used to investigate genetic relationships and the extent of redundanc...
Growth of varieties of Dioscorea alata and D. rotundata were stunted when they were grown in nutrient solutions containing 5 to 60 mg l−1 Fe. In D. alata variety TDa 95/00361, plants grown in 60 mg l−1 Fe solution exhibited reductions of 29–65% in plant height, leaf length and width, number of le...
Single node cuttings from pathogen-tested in vitro plantlets of white guinea yam (D. rotundata), cultivar TDr 93–23, were cultured in tuberization and half- and full-strength Murashige and Skoog media with 5% or 8% sucrose, with or without agar, in light or darkness and at 25 or 18°C. Microtuberi...
Low efficiency of available screening procedures constrains breeding and selection of yams (Dioscorea spp.) towards high textural quality of food products made from their tubers. This study was undertaken to determine the potential usefulness of pasting characteristics of fresh yams as rapid indi...
Yams (Dioscorea spp.) are important crops in some West African locations. The West African yam collections held at the IITA were characterized using the standard descriptor list for this crop (IPGRI/IITA. 1997. Descriptors for yam (Dioscorea spp.). International Institute of Tropical Agriculture,...
A study to examine variability in susceptibility of yams to Scutellonema bradys and to identify possible sources of resistance in Ghanaian yam germplasm (Dioscorea spp.) for use in yam improvement programmes, particularly, in West Africa was undertaken. Pot and field screening methodologies were ...
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