Immunity against Theileria parva in cattle is mediated by cytotoxic T lymphocytes (eTL) targeted at schizont-infected cells. Antigens expressed by the schizont stage of the parasite that induce protective immune response are possible candidates for sub-unit vaccine against ECF. Upon recognition a...
Bateta, R.
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[Development and optimization of enzyme linked immunoassays as readout system for activation of theileria parva-specific cytotoxic T lymphocytes.]
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Development and optimization of enzyme linked immunoassays as readout system for activation of theileria parva-specific cytotoxic T lymphocytes.
Naessens, Jan
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Newson, J.
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Bensaid, A.
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Teale, A.J.
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Magondu, J.
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Black, S.J.
,
[De novo expression of T-cell markers on Theileria parva-transformed lymphoblasts in cattle]
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De novo expression of T-cell markers on Theileria parva-transformed lymphoblasts in cattle
A murine IgM monoclonal antibody (mAb), IL-A77, has been generated that recognises the bovine transferrin receptor (TfR) and will be a useful tool to measure the activation state of bovine lymphocytes and macrophages. The antigen is detected on immature erythroid cells and proliferating lymphocyt...
Naessens, Jan
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Grab, D.J.
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Fritsch, G.
,
[Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody]
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Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody
Naessens, Jan
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Sileghem, M.R.
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MacHugh, Niall D.
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Park, Y.H.
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Davis, W.C.
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Toye, Philip G.
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[Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene]
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Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
The infection-and-treatment method (ITM) for immunization of cattle against East Coast fever has historically been used only on a limited scale because of logistical and policy constraints. Recent large-scale deployment among pastoralists in Tanzania has stimulated demand. Concurrently, a suite o...
Giulio, Giuseppe di
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Lynen, Godelieve
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Morzaria, S.P.
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Oura, C.
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Bishop, Richard P.
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[Live immunization against East Coast fever - current status]
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Live immunization against East Coast fever - current status
Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...
Bishop, Richard P.
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Shah, Tushaar
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Pelle, Roger
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Hoyle, D.
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Pearson, T.
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Haines, L.
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Brass, A.
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Hulme, H.
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Graham, S.P.
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Taracha, E.L.N.
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Lu, C.
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Hass, B.
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Wortman, J.
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White, O.
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Gardner, M.J.
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Nene, Vishvanath
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Villiers, Etienne P. de
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[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]
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Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage
The objective of this study was to investigate the relationship between seroprevalence to Theileria parva infection in cattle and potential environmental and farm-level effects in 80 farms under traditional crop–livestock system in Mbeere District, Kenya. A standardized questionnaire was used to ...
Gachohi, John M.
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Kitala, P.M.
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Ngumi, P.N.
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Skilton, Robert A.
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[Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya]
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Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant mole...
Thekisoe, O.M.M.
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Rambritch, N.E.
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Nakao, R.
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Bazie, R.S.
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Mbati, P.
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Namangala, B.
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Malele, I.
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Skilton, Robert A.
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Jongejan, F.
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Sugimoto, C.
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Kawazu, S.I.
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Inoue, N.
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[Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes]
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Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
Morrison, W. Ivan
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Goddeeris, B.M.
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[Cytotoxic T cells in immunity Therileria parva in cattle]
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Cytotoxic T cells in immunity Therileria parva in cattle
Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...
Toye, Philip G.
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Gobright, E.I.
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Nyanjui, J.
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Nene, Vishvanath
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Bishop, Richard P.
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[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]
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Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva