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In order to characterise the D17MIT16 BAC DNA clone which is part of a 1200 kilobase DNA insert believed to contain mouse trypanotolerance genes, restriction endonucleases were used to digest the insert for restriction mapping, identification of DNA markers, sub cloning and sequencing. The D17MIT...
Trypanosoma congolense, which is responsible for most of animal trypanosomiasis 10 Africa, has a great diversity of variants. Differences occur between stocks from different geographical regions and also between stocks within the same area. In the present work molecular karyotyping was used to in...
Casein kinase II from Trypanosoma congolense has been partially purified by chromatography on DEAE-cellulose and heparin-agarose and this subunit exhibits protein kinase activity with casein as the substrate. The enzyme adhered to both the DEAE-cellulose and heparin-agarose resins and was eluted ...
The studies described in this thesis were conducted to evaluate field application of the antigen-detection tube Enzyme Linked Immunosorbent Assay (ELISA) kit developed by Nantulya (1989) for diagnosis of Trypanosoma evansi infections in camels. The study was carried out on a herd of 100 camels in...
Since the anaemia of T. congolense infection, which has been shown to be mainly due to erythrocyte destruction by macrophages of the mononuclear phagocytic system, has been shown to be associated with poor reticulocyte response, a study was designed to verify if the unresponsiveness is attributab...
Argonaute proteins (AGOs) are central to RNA interference (RNAi) and related silencing pathways. At the core of the RNAi pathway in the ancient parasitic eukaryote Trypanosoma brucei is a single Argonaute protein, TbAGO1, with an established role in the destruction of potentially harmful retropos...
he many diverse and phenotypically unrelated diseases that occur with high frequency in aging mammalian species may have only a few primary causes. There is ample experimental evidence indicating that some of the essential and beneficial cellular metabolic reactions spontaneously generate free ra...
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