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A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...


Morzaria, S.P.Dolan, T.T.Norval, R.A.I.Bishop, Richard P.Spooner, P.R.[Generation and characterisation of cloned Theileria parva parasites]Generation and characterisation of cloned Theileria parva parasites
Walladde, S.M.Young, A.S.Ochieng, S.A.Mwaura, S.N.Mwakima, F.N.[Transmission of Theileria parva to cattle by Rhipicephalus appendiculatus adults fed as nymphae in vitro on infected blood through an artificial membrane]Transmission of Theileria parva to cattle by Rhipicephalus appendiculatus adults fed as nymphae in vitro on infected blood through an artificial membrane
Waladde, S.M.Young, A.S.Mwaura, S.N.Njihia, G.N.Mwakima, F.N.[Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva]Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva
Chen, P.P.Conrad, P.A.Ole-MoiYoi, O.K.Brown, W.C.Dolan, T.T.[DNA probes detect Theileria parva in the salivary glands of Rhipicephalus appendiculatus ticks]DNA probes detect Theileria parva in the salivary glands of Rhipicephalus appendiculatus ticks
Sugimoto, C.Conrad, P.A.Mutharia, L.Dolan, T.T.Brown, W.C.Pearson, T.W.Goddeeris, B.M.[Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis]Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis
Goddeeris, B.M.Katende, J.M.Irvin, A.D.Chumo, R.S.C.[Indirect fluorescent antibody test for experimental and epizootiological studies on East Coast fever (Theileria parva infection in cattle): Evaluation of a cell culture schizont antigen fixed and stored in suspension]Indirect fluorescent antibody test for experimental and epizootiological studies on East Coast fever (Theileria parva infection in cattle): Evaluation of a cell culture schizont antigen fixed and stored in suspension
Grootenhuis, J.G.Young, A.S.Stagg, D.A.Leitch, B.L.Dolan, T.T.Conrad, P.A.[Infection of African buffalo (Syncerus caffer) and cattle with Theileria parva lawrencei after serial passage in cattle]Infection of African buffalo (Syncerus caffer) and cattle with Theileria parva lawrencei after serial passage in cattle
Stagg, D.A.Conrad, P.A.Rossiter, P.B.[The isolation of Theileria parva lawrencei-infected lymphoid cell lines from free-ranging African buffaloes (Syncerus caffer)]The isolation of Theileria parva lawrencei-infected lymphoid cell lines from free-ranging African buffaloes (Syncerus caffer)
Autor: Shaw, M.K.

The main conclusion from the present study is that T. parva sporozoite entry is dependent on a functional host cell actin cytoskeleton and is not driven by the parasite. Treating lymphocytes with cytochalasin D resulted in a dose-dependent reduction in the levels of host cell infection. However, ...


Shaw, M.K.[Theileria parva: Sporozoite entry into bovine lymphocytes is not dependent on the parasite cytoskeleton]Theileria parva: Sporozoite entry into bovine lymphocytes is not dependent on the parasite cytoskeleton

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