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We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...


Ballingall, K.T.Mwangi, D.M.MacHugh, Niall D.Taracha, E.L.N.Totte, P.McKeever, Declan J.[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
Webster, P.Russo, D.C.W.Black, S.J.[The interaction of Trypanosoma brucei with antibodies to variant surface glycoproteins]The interaction of Trypanosoma brucei with antibodies to variant surface glycoproteins

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...


Kaba, S.A.Musoke, A.J.Schaap, D.Schetters, T.Rowlands, G.J.Vermeulen, A.N.Nene, VishvanathVlak, J.M.Oers, M.M. van[Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle]Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...


Lejon, VeerleClaes, F.Verloo, D.Maina, M.Urakawa, T.Majiwa, P.A.O.Buscher, P.[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels

Bovine babesiosis has been identified clinically in many areas of Morocco. A seroepidemiological survey of B. bigemina infection was conducted in the 5 main dairy cattle areas in order to estimate the prevalence of the infection. A total of 1819 bovine sera was tested using ELISA to identify ani...


El-Haj, N.Kachani, M.Ouhelli, H.Bouslikhane, M.Ahami, A.T.El-Guennouni, R.El-Hasnaoui, M.Katende, J.M.Morzaria, S.P.[Etudes epidemiologiques sur Babesia bigemina au Maroc. (Epidemiological studies of Babesia bigemina infection in Morocco)]Etudes epidemiologiques sur Babesia bigemina au Maroc. (Epidemiological studies of Babesia bigemina infection in Morocco)

Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanosoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which ...


Davison, H.C.Thrusfield, M.V.Muharsini, S.Husein, A.Partoutomo, S.Rae, P.F.Masake, R.Luckins, A.G.[Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia]Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia
Ngaira, J.M.Nantulya, V.M.Musoke, A.J.Hirumi, K.[Phagocytosis of antibody-sensitized Trypanosoma brucei in vitro by bovine peripheral blood monocytes]Phagocytosis of antibody-sensitized Trypanosoma brucei in vitro by bovine peripheral blood monocytes
Taylor, K.A.Gichuki, B.Lutje, V.Naessens, JanWilliams, D.J.L.[In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle]In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle
Pearson, T.W.Pinder, M.Roelants, G.E.Kar, S.K.Lundin, L.B.Mayor-Withey, K.S.Hewett, R.E.[Methods for derivation and detection of antiparasite monoclonal antibodies]Methods for derivation and detection of antiparasite monoclonal antibodies

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