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In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic...


Konde, V.Majiwa, P.A.O.[Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense]Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense

The extent and significance of trypanocidal drug resistance was investigated in priority areas of three East and Southern African Countries under a project. The project was built on the results of two previous projects. Existing methods currently available for the demonstration of drug-resistance...


Eisler, M.C.Ndung'u, J.M.Murilla, G.Mdachi, R.M.Mbwambo, H.Sinyangwe, L.Machila, N.Delespaux, V.Geerts, S.Brandt, J.Peregrine, A.S.McDermott, John J.Holmes, P.H.[Area-wide appraisal of drug resistance in trypanosomes infecting cattle in East and Southern Africa]Area-wide appraisal of drug resistance in trypanosomes infecting cattle in East and Southern Africa

In this study, widespread resistance of T. congolense infections in East Highland Zebu cattle at Ghibe, southwest Ethiopia, to all available trypanocidal drugs has been demonstrated. Changes in incidence of T. congolense infections in cattle 36 months and more of age sampled between 1994 and 1997...


Rowlands, G.J.[Detecting resistance of trypanosomal infection to diminazene aceturate - a possible field test?]Detecting resistance of trypanosomal infection to diminazene aceturate - a possible field test?

It is useful to be able to estimate the incidence of new infections in cattle exposed to a high challenge of drug resistance trypanosomes; with this information the impact of an intervention that improves the control of trypanosomosis can be better understood. Approximately 750 Ethiopian Highland...


Rowlands, G.J.Nagda, S.M.Leak, S.G.A.Mulatu, W.D'Ieteren, G.D.M.Peregrine, A.S.[Epidemiological analysis of trypanocidal drug resistance in Zebu cattle in Ghibe, southwest Ethiopia. Abstract]Epidemiological analysis of trypanocidal drug resistance in Zebu cattle in Ghibe, southwest Ethiopia. Abstract

To investigate the importance of trypanosomosis, resistance to trypanocidal drugs and the potential impact of integrated control measures on these factors, a 3-phase study was initiated. The first phase was a cross-sectional study in which tsetse apparent density, cattle trypanosome prevalence, t...


McDermott, John J.Sidibe, I.Bauer, B.Diarra, B.Clausen, P.H.Woitay, T.Ouédraogo, T.Kamuanga, M.Peregrine, A.S.Eisler, M.C.Mehlitz, D.[Field studies on the Development and impact of drug resistant animal trypanosomes in market oriented production systems in the Southern Guinean zone of West Africa]Field studies on the Development and impact of drug resistant animal trypanosomes in market oriented production systems in the Southern Guinean zone of West Africa

Besides the in vitro test, two tests are very commonly used for the detection of trypanocidal drug resistance: the tests in mice and in ruminants. Since it has been shown that several parameters such as the number of trypanosomes inoculated and the time of treatment might influence the outcome of...


Geerts, S.Ndung'u, J.M.Murilla, G.Mbwambo, H.Sirryangwe, L.Machila, N.Delespaux, V.Brandt, J.Peregrine, A.S.McDermott, John J.Holmes, P.H.Eisler, M.C.[In vivo tests for the detection of resistance to trypanocidal drugs: Tests in mice and in ruminants]In vivo tests for the detection of resistance to trypanocidal drugs: Tests in mice and in ruminants

The efficacy of the few available trypanocides is being seriously compromised by the emergence and spread of drug resistant trypanosome strains. Elucidation of the molecular genetic basis of this phenotype may contribute to an understanding of the mechanisms of development of resistance to the tr...


Souza, M.S. de[Analysis of two putative candidate genes for isometamidium resistance in trypanosoma congolense.]Analysis of two putative candidate genes for isometamidium resistance in trypanosoma congolense.

A deltamethrin `pour-on' insecticide was applied monthly to over 2000 cattle exposed to a high challenge of drug-resistant trypanosomes and high tsetse re-invasion pressure in the Ghibe valley, south-west Ethiopia. Blood samples were taken monthly from an average of 760 cattle for determination o...


Rowlands, G.J.Leak, S.G.A.Mulatu, W.Nagda, S.M.Wilson, A.D'Ieteren, G.D.M.[Use of deltamethrin 'pour on' insecticide for the control of cattle trypanosomiasis in the prescence of high tsetse invasion]Use of deltamethrin 'pour on' insecticide for the control of cattle trypanosomiasis in the prescence of high tsetse invasion

Bovine theileriosis caused by Theileria parva continues to be a major economic problem in many parts of Eastern, Southern, and Central Africa. Due to the unsustainable nature or the present control method-using toxic acaricides to kill ticks - alternative control methods are being sought. Live va...


Morzaria, S.P.Nene, VishvanathBishop, Richard P.Musoke, A.J.[Vaccines against Theileria parva]Vaccines against Theileria parva

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