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This protocol describes how to produce yam (Dioscorea) seeds through controlled pollination of flowers on female plants. Pollination by flower thrips takes place naturally in normal yam-growing environments in the presence of flowering male and female genotypes, although it is not efficient. This...


Mignouna, H.D.Abang, Mathew M.Asiedu, RobertGeeta, R.[Producing yam (Dioscorea) seeds through controlled crosses]Producing yam (Dioscorea) seeds through controlled crosses
Mignouna, H.D.Abang, Mathew M.Asiedu, RobertGeeta, R.[Culturing meristematic tissue and node cuttings from yams (Dioscorea)]Culturing meristematic tissue and node cuttings from yams (Dioscorea)
Mignouna, H.D.Abang, Mathew M.Asiedu, RobertGeeta, R.[True yams (Dioscorea): a biological and evolutionary link between eudicots and grasses]True yams (Dioscorea): a biological and evolutionary link between eudicots and grasses

Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the c...


Obidiegwu, J.E.Rodríguez, E.Ene-Obong, E.E.Loureiro, J.Muoneke, C.O.Santos, C.Kolesnikova-Allen, M.Asiedu, Robert[Estimation of the nuclear DNA content in some representative of genus Dioscorea]Estimation of the nuclear DNA content in some representative of genus Dioscorea

The genus Dioscorea is considered to be one of the most difficult groups for cyto-taxonomic studies because of the large number and small size of the chromosomes. The objective of the present study was to develop an efficient method of chromosome observation by identifying the optimum time for co...


Babil, P.K.Kikuno, H.Shiwachi, H.Toyohara, H.Fujigaki, J.Fujimaki, H.Asiedu, Robert[Optimum time for collection of root samples for chromosome observation in yams (Dioscorea spp.)]Optimum time for collection of root samples for chromosome observation in yams (Dioscorea spp.)

Diagnosis of virus diseases of yams at the International Institute of Tropical Agriculture (IITA) is based on the use of indicator plants, insect vectors, serological diagnostics, and electron microscopy (EM). Yam I (YVI), yam mosaic (YMV), and Dioscorea dumetorum (DdV) potyviruses even in mixed ...


Hughes, J.Dongo, L.Ng, S.Y.C.[Diagnosis of yam viruses]Diagnosis of yam viruses

The response of yam (Dioscorea) cultivars to artificial and natural infections with minisett rot pathogens was investigated. None of the 20 yam cultivars were resistant to Sclerotium rolfsii [Corticium rolfsii] or Trichoderma longibrachyatum. Cultivars TDa 84/093, 85/236, 85/257, TDr 608, 745, 81...


Osai, E.O.Ikotun, T.Asiedu, RobertHahn, S.K.[Susceptibility of yam cultivars to minisett rot pathogens]Susceptibility of yam cultivars to minisett rot pathogens
Mignouna, H.Asiedu, RobertThottappilly, G.Hahn, S.K.[Genetic diversity and phylogenetic relationships in the genus Dioscorea using PCRRAPD]Genetic diversity and phylogenetic relationships in the genus Dioscorea using PCRRAPD

Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA...


Eni, A.Hughes, J.Asiedu, RobertRey, M.[Re-evaluation of Yam Mosaic Virus (YMV) detection methods]Re-evaluation of Yam Mosaic Virus (YMV) detection methods

Aims: To study yam propagation and seed yam tuber production in aeroponics system.Study Design: The experiment was conducted in a randomized complete block designand treatments were replicated three times.Place of Study: This experiment was carried out at the International Institute of TropicalAg...


Maroya, N.Balogun, M.Asiedu, RobertAighewi, B.A.Kumar, P.L.Augusto, J.[Yam propagation using Aeroponics technology]Yam propagation using Aeroponics technology

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