Sensitivity and specificity of the FAO/IAEA kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (three populations) or T. vivax (one population). The kits are based on mono...
Eisler, M.C.
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Lessard, P.
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Masake, R.A.
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Moloo, S.K.
,
Peregrine, A.S.
,
[Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle]
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Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle
Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...
Honda, Y.
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Matsubara, Y.
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Morzaria, S.P.
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McKeever, Declan J.
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[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]
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Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes
Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recentl...
Totte, P.
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Bensaid, A.
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Mahan, S.M.
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Martínez, D.
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McKeever, Declan J.
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[Immune responses to Cowdria ruminantium infections]
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Immune responses to Cowdria ruminantium infections
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...
Ballingall, K.T.
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Mwangi, D.M.
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MacHugh, Niall D.
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Taracha, E.L.N.
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Totte, P.
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McKeever, Declan J.
,
[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]
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A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
Gee, A.L.W. de
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[Host-parasite relationships in Trypanosoma (Duttonella ) vivax with special reference to the influence of antigenic variation]
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Host-parasite relationships in Trypanosoma (Duttonella ) vivax with special reference to the influence of antigenic variation
East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...
Waithanji, Elizabeth M.
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Nantulya, E.M.
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Mbiuki, S.M.
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[Use of antigens capture tube enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma evansi infections in dromedary camels (Camelus dromedarius)]
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Use of antigens capture tube enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma evansi infections in dromedary camels (Camelus dromedarius)
Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanosoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which ...
Davison, H.C.
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Thrusfield, M.V.
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Muharsini, S.
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Husein, A.
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Partoutomo, S.
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Rae, P.F.
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Masake, R.
,
Luckins, A.G.
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[Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia]
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Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia