A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous control, has been developed and validated by four National Reference Laboratories of the European Union for Af...
Tignon, M.
,
Gallardo, C.
,
Iscaro, C.
,
Hutet, E.
,
Stede, Y. van der
,
Kolvasov, D.
,
Mia, G.M. de
,
Potier, M.F. le
,
Bishop, Richard P.
,
Arias, Marisa
,
Koenen, F.
,
[Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus]
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Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus
Balcázar, María del Socorro
,
Cuervo Ibáñez, Maritza
,
[Implementación de una técnica molecular de diagnóstico mediante la reacción en cadena de la polimerasa (PRC) para la detección de la bacteria Curtobacterium flaccumfasciens pv. flaccumfasciens (Cff) en semillas analizada en el laboratorio de sanidad de germoplasma del CIAT.]
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Implementación de una técnica molecular de diagnóstico mediante la reacción en cadena de la polimerasa (PRC) para la detección de la bacteria Curtobacterium flaccumfasciens pv. flaccumfasciens (Cff) en semillas analizada en el laboratorio de sanidad de germoplasma del CIAT.
Salcedo, A
,
Mancilla, LI
,
Cortés Bermúdez, D.F.
,
Beeching, JR
,
Chavarriaga Aguirre, Paul
,
Tohme, Joseph M.
,
[Cloning carotene synthesis genes from cassava roots]
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Cloning carotene synthesis genes from cassava roots
Echeverry Solarte, Morgan
,
Marcilla, Lida Inés
,
Cortéz, Diego Fernando
,
Chavarriaga Aguirre, Paul
,
Tohme, Joseph M.
,
[Evaluación preliminar de la expresión del gen bar en plantas transgénicas de yuca (Manihot esculenta) mantenidas en reproducción vegetativa por cerca de diez años]
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Evaluación preliminar de la expresión del gen bar en plantas transgénicas de yuca (Manihot esculenta) mantenidas en reproducción vegetativa por cerca de diez años
Thottappilly, G.
,
Dahal, G.
,
Harper, G.
,
Hull, R.
,
Lockhart, B.
,
[Banana streak badnavirus: development of diagnostics by ELISA and PCR]
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Banana streak badnavirus: development of diagnostics by ELISA and PCR
Andean potato mottle virus (APMoV, genero Comovirus) es un patogeno importante de varias plantas Solanaceas, que incluyen papa, tomate y tabaco, pero hasta la fecha solo esta disponible datos de secuencias parciales. En este estudio, se determino el genoma completo de APMoV utilizando secuenciami...
Souza, J. de
,
Muller, G.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.]
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Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.
El virus colusivo del camote (SPCV, genero Cavemovirus), el virus del aclaramiento de venas del camote (SPVC, genero Solendovirus) y el virus del enrollamiento de hojas del camote (SPLCV, genero Begomovirus) son virus con genoma de ADN, presentes en el camote en infecciones virales simples o mult...
Berrocal, A.
,
Rossel, G.
,
Fuentes, S.
,
Pérez, A.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)]
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Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)
Kreuze, J.F.
,
Koening, R
,
Souza, J. de
,
Vetten, H.J.
,
Muller, G
,
Flores, B.
,
Ziebell, H
,
Cuéllar, Wilmer J.
,
[The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species]
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The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species
Phenylalanine Ammonia Lyase (PAL) gene which plays a key role in bio-synthesis of medicinally important compounds, Rutin/quercetin was sequence characterized for its efficient genomics application. These compounds possessing anti-diabetic and anti-cancer properties and are predominantly produced ...
Thiyagarajan, K.
,
Vitali, F.
,
Tolaini, V.
,
Galeffi, P.
,
Cantale, C.
,
Vikram, P.
,
[Genomic characterization of Phenylalanine ammonia lyase gene in buckwheat]
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Genomic characterization of Phenylalanine ammonia lyase gene in buckwheat
Bacterial wilt caused by Ralstonia solanacearum is considered among the most damaging diseases of potato in Sub-Saharan Africa and the most significant biotic constraint of potato production alongside late blight. Unlike late blight, which can be managed by chemical means, R. solanacearum can onl...
Okiro, L.A.
,
Tancos, M.A.
,
Nyanjom, S.G.
,
Smart, C.D.
,
Parker, M.
,
[Comparative evaluation of LAMP, qPCR, conventional PCR, and ELISA to detect ralstonia solanacearum in Kenyan potato fields.]
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Comparative evaluation of LAMP, qPCR, conventional PCR, and ELISA to detect ralstonia solanacearum in Kenyan potato fields.