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A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported pre...


Hussain, A.Espinel, MTRoca, William M.Bushhuk, W[Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)]Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)

Mammary gland is an important organ for milk synthesis and secretion. It undergoes dramatic physiological changes to adapt the shift from peak to late lactation stage. Protein plays a final very vital role in many life functions, and the protein changes during different lactation stages potential...


Zheng, XianruiNing, ChaoDong, YichunZhao, PengjuLi, JunhuiZiyao FanLi, JiangYing YuMrode, Raphael A.Liu, Jian Feng[Quantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stages]Quantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stages

Sweetpotato (Ipomoea batatas L.(LAM)) is one of the most important food crops in developing countries, including South Africa. Most sweetpotato varieties in South Africa are cream or white fleshed and lack the essential beta-carotene of the orange-fleshed varieties. To develop new varieties with ...


Selaocoe, M.E.Adebola, P.Pillay, M.Laurie, S.M.[Genetic diversity of South African sweetpotato germplasm using molecular markers]Genetic diversity of South African sweetpotato germplasm using molecular markers

The nutritional quality of extruded unmalted or malted maize fortified with cowpea as complementary food was assessed based on its proximate analysis, amino acid composition and results from rat feeding with the blends. Results indicated a slight decrease and increase in protein content due to ma...


Obatolu, V.Cole, A.Maziya-Dixon, B.B.[Nutritional quality of complementary food prepared from unmalted and malted maize fortified with cowpea using extrusion cooking]Nutritional quality of complementary food prepared from unmalted and malted maize fortified with cowpea using extrusion cooking

We initiated a proteomics-based approach to identify root proteins affected by salinity in pea (Pisum sativum cv. Cutlass). Salinity stress was imposed either on 2-wk old pea plants by watering with salt water over 6 wk or by germinating and growing pea seeds for 7 days in Petri dishes. Concentra...


Kav, N.N.Srivastava, S.Goonewardene, L.Blade, S.F.[Proteomelevel change in the roots of Pisum sativum L. in response to salinity]Proteomelevel change in the roots of Pisum sativum L. in response to salinity
Savadye, D.T.[Establishment of an expressed sequence-tag database,mapping of theileria parva schizont cDNAs and molecular characterisation of a parasite-specific protein.]Establishment of an expressed sequence-tag database,mapping of theileria parva schizont cDNAs and molecular characterisation of a parasite-specific protein.
Kwarteng, T.A.[Protein phosphorylation and differentation in bloodstream forms of trypanosoma brucei brucei.]Protein phosphorylation and differentation in bloodstream forms of trypanosoma brucei brucei.

Procedures for the electrophoretic detection of isoenzymes and other proteins contained in cassava, beans, and legumes are described. Diagrams illustrating the construction and assembly of equipment required to conduct starch gel and polyacrylamide gel electrophoresis and protein electrophoresis ...

Se describen los procedimientos para la deteccion electroforetica de isoenzimas y proteinas presentes en materiales de yuca, frijol y leguminosas. Se detallan por medio de graficas la construccion y ensamble del equipo necesario para llevar a cabo electroforesis con gel de almidon y con gel de po...


Hussain, A.Ramírez, H.Roca, William M.[Manual practico para la deteccion electroforetica de isoenzimas y otras proteinas]Manual practico para la deteccion electroforetica de isoenzimas y otras proteinas
Autor: Lareo, LR

The low digestibility of grain legumes, particularly beans, is highlighted and its regulating factors (tannins, seed coat fiber, protease inhibitors, protein structure of the protein to digest, interactions between proteins and other cotyledon nutrients, etc.) are explained with the aid of a line...


Lareo, LR[Digestibilidad de las proteinas de frijol factores reguladores]Digestibilidad de las proteinas de frijol factores reguladores

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