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Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived small interfering RNAs has proven to be a highly efficient approach for virus discovery. Here we present VirusDetect, a bioinformatics pipelin...


Yi ZhengShan GaoPadmanabhan, C.Li, R.Galvez, M.Gutiérrez, D.Fuentes, S.Kai-Shu LingKreuze, J.F.Zhangjun Fei[VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.]VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.

In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this st...


Bömer, M.Rathnayake, A.I.Visendi, P.Sewe, S.O.Sicat, J.P.A.Silva, G.Kumar, P.L.Seal, S.E.[Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses]Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses

Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt...


Wagaba, HenryPatil, Basavaprabhu L.Mukasa, SettumbaAlicai, TitusFauquet, Claude M.Taylor, Nigel J.[Artificial microRNA-derived resistance to Cassava brown streak disease]Artificial microRNA-derived resistance to Cassava brown streak disease

Prentice, K.Pertry, I.Christiaens, O.Bauters, L.Bailey, M.Niblett, C.Ghislain, M.Gheysen, G.Smagghe, G.[Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae]Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae

Drought stress is one of the major abiotic factors limiting banana (Musa) production. Even mild-drought conditions are responsible for considerable yield losses. We performed large-scale transcriptome sequencing using Illumina technology on root tissue of three triploid genotypes representing wel...


Zorrilla-Fontanesi, Y.Rouard, M.Cenci, A.Kissel, E.Do, H.Dubois, E.Nidelet, S.Roux, N.Swennen, R.L.Carpentier, S.C.[Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip]Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip

Sarah, GautierHoma, FelixPointet, StéphanieContreras, SandySabot, FrançoisNabholz, BenoitSantoni, SylvainSauné, LaureArdisson, MorganeChantret, NathalieSauvage, ChristopherTregear, JamesJourda, CyrilPot, DavidVigouroux, YvesChair, HanaScarcelli, NoraBillot, ClaireYahiaoui, NabilaBacilieri, RobertoKhadari, BouchaibBoccara, MichelBarnaud, AdélinePéros, Jean-PierreLabouisse, Jean-PierrePham, Jean-LouisDavid, JacquesGlémin, SylvainRuiz, Manuel[A large set of 26 new reference transcriptomes dedicated to comparative population genomics in crops and wild relatives]A large set of 26 new reference transcriptomes dedicated to comparative population genomics in crops and wild relatives

Thrips tabaci Lindeman is an important polyphagous insect pest species estimated to cause losses of more than U.S. $1 billion worldwide annually. Chemical insecticides are of limited use in the management of T. tabaci due to the thigmokinetic behavior and development of resistance to insecticides...


Balan, R.K.Ramasamy, A.Hande, R.H.Gawande, S.J.Krishna Kumar, N.K.[Genome-wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips, Thrips tabaci Lindeman (Thysanoptera:Thripidae), vectors of tospoviruses (Bunyaviridae)]Genome-wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips, Thrips tabaci Lindeman (Thysanoptera:Thripidae), vectors of tospoviruses (Bunyaviridae)

Cenci, A.Zorrilla-Fontanesi, J.Kissel, E.Hueber, Y.Dubois, E.Parrinello, H.Swennen, R.L.Roux, N.Rouard, M.Carpentier, S.[Transcriptomic analysis in banana cultivars during an early stage of drought-stress]Transcriptomic analysis in banana cultivars during an early stage of drought-stress
Rodríguez, F.Ballén, CAyling, S.Plata, GTohme, Joseph M.[Predicción de MicroRNAs asociados a respuesta a sequía en yuca (Manihot esculenta Crantz)]Predicción de MicroRNAs asociados a respuesta a sequía en yuca (Manihot esculenta Crantz)

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