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East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...


Kaba, S.A.Schaap, D.Roode, E.C.Nene, VishvanathMusoke, A.J.Vlak, J.M.Oers, M.M. van[Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens]Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens

Recent studies suggest that peptidyl-prolyl isomerases of the cyclophilin family, that access the secretory pathway, can be involved in the interaction of parasitic protozoa with mammalian host cells. The amino acid sequence of a cDNA encoding a cyclophilin family member of the intracellular pro...


Ebel, T.Pelle, RogerJanoo, R.T.K.Lipp, J.Bishop, Richard P.[A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum]A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

The association between mean daily weight gain, Theileria parva infections, clinical East Coast fever and other possible determinants of weight gain were examined in a longitudinal observational study that was conducted in cohorts of female calves from five agro-ecological zone (AEZ)-grazing stra...


Gitau, G.K.McDermott, John J.McDermott, B.Perry, Brian D.[The impact of Theileria parva infections and other factors on calf mean daily weight gains in smallholder dairy farms in Murang'a District]The impact of Theileria parva infections and other factors on calf mean daily weight gains in smallholder dairy farms in Murang'a District

A clinical trial, comparing East Coast fever (ECF) control strategies in 5 different groups of traditionally managed Sanga cattle (44-49 animals per herd), was conducted in Zambia over 2.5 years between 1992 and 1995. Two groups were kept under intensive tick control by weekly cypermethrin (Barri...


Minjauw, B.Otte, M.J.James, A.D.Castro, J.J. deSinyangwe, P.[Effect of different East Coast Fever control strategies on disease incidence in traditionally managed Sanga cattle in Central Province of Zambia]Effect of different East Coast Fever control strategies on disease incidence in traditionally managed Sanga cattle in Central Province of Zambia
Pain, A.Renauld, H.Berriman, M.Murphy, L.Yeats, C.A.Weir, W.Kerhornou, A.Aslett, M.Bishop, Richard P.Bouchier, C.Bouchier, C.Cochet, M.Coulson, R.M.R.Cronin, A.Villiers, Etienne P. deFraser, A.Fosker, N.Gardner, M.Goble, A.Griffiths-Jones, S.Harris, D.E.Katzer, F.Larke, N.Lord, A.Maser, P.McKellar, S.Mooney, P.Morton, F.Nene, VishvanathO'Neil, S.Price, C.Quail, M.A.Rabbinowitsch, E.Rawlings, N.D.Rutter, S.Saunders, D.Seeger, K.Shah, TushaarSquares, R.Squares, S.Tivey, A.Walker, A.R.Woodward, J.Dobbelaere, D.A.E.Langsley, G.Rajandream, M.A.McKeever, Declan J.Shiels, B.Tait, A.Barrell, B.Hall, N.[Genome of the host-cell transforming parasite Theileria annulata compared with T. parva]Genome of the host-cell transforming parasite Theileria annulata compared with T. parva

The effects of holding temperature, pH and medium on the infectivity of Theileria parva sporozoites were investigated using an in vitro infectivity assay. The sporozoite infectivity lasted for 72 h at a holding temperature of 4 C but for only 24 h at 24 degrees C. Sporozoite infectivity was fo...


Kimbita, E.N.Silayo, R.S.Dolan, T.T.[Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity]Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity

Among the blood-sucking arthropods, ixodid ticks have some of the most complicated feeding behavior requirements. Feeding is preceded by an attachment process, and each instar has to remain fully attached to the same feeding site for a period of 2-14 days. These biological demands have for a long...


Young, A.S.Waladde, S.M.Morzaria, S.P.[Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission]Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission
Ole-MoiYoi, O.K.Nayar, A.Iams, K.P.Musoke, A.J.Yilma, T.[Molecular aspects of Theileria parva and approaches to vaccine development for animals]Molecular aspects of Theileria parva and approaches to vaccine development for animals
Autor: Shaw, M.K.

The role of calcium in Theileria parva sporozoite entry into bovine lymphocytes was examined. Depletion of Ca2+ from the external medium had little effect on sporozoite entry suggesting that the initial sporozoite-host cell interaction is a Ca2+-independent process. Sporozoite entry could, howeve...


Shaw, M.K.[Mobilization of intrasporozoite Ca(2+) is essential for Theileria parva sporozoite invasion of bovine lymphocytes]Mobilization of intrasporozoite Ca(2+) is essential for Theileria parva sporozoite invasion of bovine lymphocytes

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