The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells prolifer...
Totte, P.
,
McKeever, Declan J.
,
Jongejan, F.
,
Barbet, A.
,
Mahan, S.M.
,
Mwangi, D.
,
Bensaid, A.
,
[Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium]
,
Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium
This paper determines whether contact between parasite-specific CD4+ T cells and CD8+ T cells is an essential requirement for helper function, additional experiments were performed using two-chamber culture plates incorporating semi-porous membranes. TpM were co-cultured with naive or immune CD8 ...
Taracha, E.L.N.
,
McKeever, Declan J.
,
[Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes]
,
Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes
Techniques for in vivo depletion of lymphocytes using monoclonal antibodies have been well documented for murine systems. This model has played an important role in analysing the involvement of various lymphocyte subsets in several immune mechanisms. In mice 600 ug of Ab are generally injected in...
Analysis of serum antibody responses in cattle experimentally infected with Trypanosoma congolense suggested that antibody responses of trypanosusceptible Boran cattle are less efficient in isotype switching and affinity maturation than those of trypanotolerant N'Dama cattle. High levels of polys...
Peptide-major histocompatibility complex (p-MHC) class I tetramer complexes have facilitated the early detection and functional characterisation of epitope specific CD8+ cytotoxic T lymphocytes (CTL). Here, we report on the generation of seven recombinant bovine leukocyte antigens (BoLA) and reco...
Svitek, Nicholas
,
Hansen, A.M.
,
Steinaa, Lucilla
,
Saya, R.
,
Awino, Elias
,
Nielsen, Morten
,
Buus, Soren
,
Nene, Vishvanath
,
[Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle]
,
Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle
Naessens, Jan
,
Howard, C.J.
,
[Monoclonal antibodies reacting with bovine B cells (BoWC3, BoWC4 and BoWC5)]
,
Monoclonal antibodies reacting with bovine B cells (BoWC3, BoWC4 and BoWC5)
Muiya, P.
,
Logan-Henfrey, L.L.
,
Naessens, Jan
,
[Expression of antigens on haemopoietic progenitor cells in bovine bone marrow]
,
Expression of antigens on haemopoietic progenitor cells in bovine bone marrow
International Laboratory for Research on Animal Diseases
,
[ILRAD Reports. Volume 9. Number 1. January 1991]
,
ILRAD Reports. Volume 9. Number 1. January 1991