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Martínez Racines, César PompilioArumuganathan, K.Kikuchi, H.Earle, E.D.[Estimation of nuclear DNA content in Oryza to flow cytometry]Estimation of nuclear DNA content in Oryza to flow cytometry

A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoame...


Carvajal-Yepes, MonicaZambrano, LeidyBueno, Juan MiguelRaatz, BodoCuéllar, Wilmer J.[Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia]Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia

Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguic...


Ocampo Nahar, César H.Martin, JPSánchez Yélamo, María DoloresOrtiz, J.M.Toro Chica, Orlando[Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers]Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers

We determined DNA fingerprints and pathotypes on international differentials for 151 isolates of the rice blast fungus Pyricularia grisea that were collected from 15 rice cultivars at a blast disease breeding nursery in Santa Rosa, Colombia. The pathogen population expressed an extraordinarily hi...


Levy, MCorrea Victoria, Fernando J.Zeigler, Robert S.Xu, SHamer, JE[Genetic diversity of the rice blast fungus in a disease nursery in Colombia]Genetic diversity of the rice blast fungus in a disease nursery in Colombia
Asfaw Amele, A.Blair, Matthew W.Almekinders, Conny J.M.[Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands]Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands

Bean golden mosaic geminivirus (BGMV) causes major losses on beans (Phaseolus vulgaris) in the tropical and subtropical Americas and the Caribbean Basin. Infectious DNA-A and -B components of BGMV isolates from the Dominican Republic (BGMV-DR) and Guatemala (BGMV-GA) were sequenced, and their gen...


Faria, J.C. deGilbertson, R.L.Hanson, SFMorales, Francisco JoséAhlquist, P.G.Loniello, AOMaxwell, D.P.[Bean golden mosaic geminivirus type II isolates from the Dominican Republic and Guatemala : nucleotide sequences, infectious pseudorecombinants, and phylogenetic relationships]Bean golden mosaic geminivirus type II isolates from the Dominican Republic and Guatemala : nucleotide sequences, infectious pseudorecombinants, and phylogenetic relationships

Geminiviruses are small plant viruses that encapsidate a single-stranded circular DNA and form double-stranded DNAs in infected plants. Infection of economically important hosts with cloned DNAs of geminiviruses frequently has been difficult. Four isolates of bipartite bean-infecting geminiviruse...


Gilbertson, R.L.Faria, J.C. deHanson, SFMorales, Francisco JoséAhlquist, P.G.Maxwell, D.P.Russell, DR[Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration]Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration

Breeding of Phaseolus vulgaris L. for resistance to common bacterial blight (CBB) can be done with visual evaluations of symptoms to distinguish broad resistance classes, but a more quantitative measure was needed for genetic studies of resistance. A novel method of evaluation was developed by qu...


Constable, ECMichaelis, TEGoodwin, PHMayer, Jorge EdgardPastor Corrales, Marcial A.[Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a breeding program]Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a breeding program

DNA polymorphism and variation in virulence of Xanthomonas axonopodis pv. manihotis (Xam), the causal agent of cassava bacterial blight, were studied within a pathogen population from Venezuela. Collections were made in several fields at different sites within an edaphoclimatic zone where cassava...


Verdier, ValérieRestrepo, S.Mosquera Cifuentes, Gloria MariaDuque E., Myriam CristinaGerstl, ALaberry Saavedra, Rafael Alberto[Genetic and pathogenic variation of Xanthomonas axonopodis pv. manihotis in Venezuela]Genetic and pathogenic variation of Xanthomonas axonopodis pv. manihotis in Venezuela

Chloroplast DNA polymorphisms were studied by PCR sequencing and PCR-restriction fragment length polymorphism in 165 accessions of domesticated landraces of common bean from Latin America and the USA, 23 accessions of weedy beans, and 134 accessions of wild beans covering the entire geographic ra...


Chacón Sánchez, María I.Pickersgill, BDebouck, Daniel G.[Domestication patterns in common bean (Phaseolus vulgaris L.) and the origin of the Mesoamerican and Andean cultivated races]Domestication patterns in common bean (Phaseolus vulgaris L.) and the origin of the Mesoamerican and Andean cultivated races

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