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The infection-and-treatment method (ITM) for immunization of cattle against East Coast fever has historically been used only on a limited scale because of logistical and policy constraints. Recent large-scale deployment among pastoralists in Tanzania has stimulated demand. Concurrently, a suite o...


Giulio, Giuseppe diLynen, GodelieveMorzaria, S.P.Oura, C.Bishop, Richard P.[Live immunization against East Coast fever - current status]Live immunization against East Coast fever - current status

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

The objective of this study was to investigate the relationship between seroprevalence to Theileria parva infection in cattle and potential environmental and farm-level effects in 80 farms under traditional crop–livestock system in Mbeere District, Kenya. A standardized questionnaire was used to ...


Gachohi, John M.Kitala, P.M.Ngumi, P.N.Skilton, Robert A.[Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya]Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya

We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant mole...


Thekisoe, O.M.M.Rambritch, N.E.Nakao, R.Bazie, R.S.Mbati, P.Namangala, B.Malele, I.Skilton, Robert A.Jongejan, F.Sugimoto, C.Kawazu, S.I.Inoue, N.[Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes]Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...


Sohanpal, B.K.Morzaria, S.P.Gobright, E.I.Bishop, Richard P.[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome
Baldwin, C.L.Malu, M.N.Grootenhuis, J.G.[Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva]Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
Emery, D.L.MacHugh, Niall D.Morrison, W. Ivan[Theileria parva (Muguga) infects bovine T-lymphocytes in vivo and induces coexpression of BoT4 and BoT8]Theileria parva (Muguga) infects bovine T-lymphocytes in vivo and induces coexpression of BoT4 and BoT8
Allsopp, B.A.Carrington, M.Baylis, H.Sohal, S.Dolan, T.T.Iams, K.P.[Improved characterization of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes]Improved characterization of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes

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