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Background Therecent development and availability of different genotype by sequencing (GBS) protocols provided a cost-effective approach to perform high-resolution genomic analysis of entire populations in different species. The central component of all these protocols is the digestion of the ini...


Perea, ClaudiaHoz, Juan Fernando de laCruz, Daniel FelipeLobaton, Juan DavidIzquierdo, PauloQuintero, Juan CamiloRaatz, BodoDuitama, Jorge[Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP]Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP
Utsumi, YoshinoriTanaka, MahoKurotani, AtsushiYoshida, TakuhiroMochida, KeiichiMatsui, AkihiroIshitani, ManabuSraphet, SupajitWhankaew, SukhumanAsvarak, ThipaNarangajavana, JarunyaTriwitayakorn, KanokpornSakurai, TetsuyaSeki, Motoaki[Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray]Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
Hoyos, R.A.Roca, William M.Hosfield, GLMayer, Jorge Edgard[Particle gun transient expression of sexual embryos of common bean]Particle gun transient expression of sexual embryos of common bean
Afanador Kafury, LucíaHaley, SDKelly, James D.[Adoption of a "mini-prep" DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.)]Adoption of a "mini-prep" DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.)

The Chilean national common bean germplasm collection consists of 1200 accessions, most of which have not been characterized or used in breeding programs. As part of the characterization of this germplasm we have formed a core collection of 246 accessions that represent 21% of the whole collectio...


Becerra Velásquez, Viviana L.Paredes, MRojo, CDíaz, Lucy MilenaBlair, Matthew W.[Microsatellite marker characterization of Chilean Common Bean (Phaseolus vulgaris L.) germplasm]Microsatellite marker characterization of Chilean Common Bean (Phaseolus vulgaris L.) germplasm

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

The wild common bean (Phaseolus vulgaris) is widely but discontinuously distributed from northern Mexico to northern Argentina on both sides of the Isthmus of Panama. Little is known on how the species has reached its current disjunct distribution. In this research, chloroplast DNA polymorphisms ...


Chacón Sánchez, María I.Pickersgill, BDebouck, Daniel G.Arias, J.S.[Phylogeographic analysis of the chloroplast DNA variation in wild common bean (Phaseolus vulgaris L.) in the Americas]Phylogeographic analysis of the chloroplast DNA variation in wild common bean (Phaseolus vulgaris L.) in the Americas
Wenzl, PeterMancilla, Lida I.Rao, Idupulapati M.Mayer, Jorge Edgard[Isolation of rare cDNAs by asymmetric self-hybridization]Isolation of rare cDNAs by asymmetric self-hybridization

Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this...


Chavez, Alba L.Vergara, CEMayer, Jorge Edgard[Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues]Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues

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