Bloodstream forms (BSF) and procyclic culture forms (PCF) of African trypanosomes were incubated with a variety of lectins in vitro. Cessation of cell division and profound morphological changes were seen in procyclic forms but not in BSF after incubation with concanvavalin A (Con A). Wheat germ...
Pearson, T.W.
,
Beecroft, R.P.
,
Welburn, S.C.
,
Ruepp, S.
,
Roditi, I.
,
Kuo-Yuan, H.
,
Englund, P.T.
,
Wells, C.W.
,
Murphy, N.B.
,
[The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro]
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The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro
Four monoclonal antibodies were produced against endozoite membrane and cytoplasmic antigens of B. besnoiti. In immunofluorescence antibody tests, three of the clones, designated 2M3C5, 2M1G8 and 2M9G3 recognized antigens restricted to the anterior pole of the endozoites. The fourth clone, 2M9C4,...
The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....
Bishop, Richard P.
,
Geysen, D.
,
Spooner, P.R.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]
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Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle
Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...
Honda, Y.
,
Matsubara, Y.
,
Morzaria, S.P.
,
McKeever, Declan J.
,
[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]
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Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes
Diall, O.
,
Nantulya, V.M.
,
Luckins, A.G.
,
Diarra, B.
,
Kouyate, B.
,
[Evaluation on mono-and polyclonal antibody-based antigen detection immunoassays for diagnosis of Trypanosoma evansi infection in the dromedary camel]
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Evaluation on mono-and polyclonal antibody-based antigen detection immunoassays for diagnosis of Trypanosoma evansi infection in the dromedary camel
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Nantulya, V.M.
,
Lindqvist, K.J.
,
Stevenson, P.
,
Mwangi, E.K.
,
[Application of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay for field diagnosis of bovine trypanosomiasis at Nguruman, Kenya]
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Application of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay for field diagnosis of bovine trypanosomiasis at Nguruman, Kenya
Nantulya, V.M.
,
Bajyana Songa, E.
,
Hamers, R.
,
[Detection of circulating trypanosomal antigen in Trypanosoma evansi-infected animals using a Trypanosoma brucei group-specific monoclonal antibody]
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Detection of circulating trypanosomal antigen in Trypanosoma evansi-infected animals using a Trypanosoma brucei group-specific monoclonal antibody
Mice were immunized with either an isometamidium-human serum albumin (HSA) conjugate or an isometamidium-porcine thyroglobulin conjugate (PTG). Thereafter, monoclonal antibodies (MAbs) IL-A 1001, IL-A 1002, IL-A 1003, 5F7.B7, and 5F7.C9 were generated and selected on the basis that they recognize...
Peregrine, A.S.
,
Eisler, M.C.
,
Katende, J.
,
Flynn, J.N.
,
Gault, E.A.
,
Kinabo, L.D.B.
,
Holmes, P.H.
,
[Generation of monoclonal antibodies to the anti-trypanosomal drug isometamidium]
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Generation of monoclonal antibodies to the anti-trypanosomal drug isometamidium
Davis, W.C.
,
Ellis, J.A.
,
MacHugh, Niall D.
,
Baldwin, C.L.
,
[Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2]
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Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2