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There is growing evidence that immunity of cattle to Cowdria ruminantium infection is mediated by T lymphocytes. C. ruminantium antigens that stimulate these responses are therefore of considerable importance to the development of a sub-unit vaccine against the disease. We have examined T cell re...


Mwangi, D.M.McKeever, Declan J.Nyanjui, J.K.Barbet, A.F.Mahan, S.M.[Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent]Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent

Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation i...


Urakawa, T.Majiwa, P.A.O.[Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense]Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
Shapiro, S.Z.Young, J.R.[An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen]An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen

Cassava brown streak disease (CBSD) threatens food and economic security for smallholder farmers throughout East and Central Africa, and poses a threat to cassava production in West Africa. CBSD is caused by two whitefly-transmitted virus species: Cassava brown streak virus (CBSV) and Ugandan cas...


Beyene, GetuChauhan, Raj DeepikaIlyas, MuhammadWagaba, HenryFauquet, Claude M.Miano, DouglasAlicai, TitusTaylor, Nigel J.[A Virus-derived stacked RNAi construct confers robust resistance to cassava brown streak disease]A Virus-derived stacked RNAi construct confers robust resistance to cassava brown streak disease

BACKGROUND. RNA interference (RNAi) technology can potentially serve as a suitable strategy to control the African sweet potato weevil Cylas puncticollis (SPW), which is a critical pest in sub-Saharan Africa. Important prerequisites are required to use RNAi in pest control, such as the presence o...


Prentice, K.Christiaens, O.Pertry, I.Bailey, M.Niblett, C.Ghislain, M.Gheysen, G.Smagghe, G.[RNAi-based gene silencing through dsRNA injection or ingestion against the African sweet potato weevil Cylas puncticollis (Coleoptera: Brentidae).]RNAi-based gene silencing through dsRNA injection or ingestion against the African sweet potato weevil Cylas puncticollis (Coleoptera: Brentidae).

The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...


Bishop, Richard P.Gobright, E.I.Spooner, P.R.Allsopp, B.A.Sohanpal, B.K.Collins, N.[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva

Procyclic Trypanosoma brucei rhodesiense have a cell death mechanism which can be activated by an external signal, the lectin ConA, in vitro. ConA has been shown to cause profound changes in cellular morphology and induce fragmentation of nuclear DNA in T.b. rhodesiense which are characteristic o...


Murphy, N.B.Welburn, S.C.[Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs]Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs
Autor: Goldman, M.

In this paper I argue for building dialogues between scientific and Maasai knowledge articulations, utilizing knowledge of wildebeest as an example. By locating Maasai and scientific knowledges regarding a particular subject (wildebeest) in relation to each other-in discourse and in practice-my i...


Goldman, M.[Tracking wildebeest, locating knowledge: Maasai and conservation biology understandings of wildebeest behavior in northern Tanzania]Tracking wildebeest, locating knowledge: Maasai and conservation biology understandings of wildebeest behavior in northern Tanzania
Pelle, RogerMurphy, N.B.[In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA]In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA

The cDNA coding for the soluble form of bovine stem cell factor (boSCF-Ala-105) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. ...


Mertens, B.Muriuki, C.Muiya, P.Andrianarivo, A.Mwangi, S.Logan-Henfrey, L.L.[Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense]Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense

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