Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
,
Morzaria, S.P.
,
Toye, Philip G.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Nkonge, C.
,
Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
,
An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
An outbreak of East Coast fever (ECF) occurred in an experimental herd of Sanga cattle maintained under a traditional rangeland grazing system at Lutale, Central Province of Zambia. Two groups of cattle had been kept under different tick-control regimes for several years prior to the introduction...
Minjauw, B.
,
Otte, M.J.
,
James, A.D.
,
Castro, J.J. de
,
Permin, A.
,
Giulio, Giuseppe di
,
[An outbreak of East Coast Fever in a herd of Sanga cattle in Lutale, Central Province of Zambia]
,
An outbreak of East Coast Fever in a herd of Sanga cattle in Lutale, Central Province of Zambia
The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....
Bishop, Richard P.
,
Geysen, D.
,
Spooner, P.R.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]
,
Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle
Interviews with all the households in the Agropastoral Zone (ZAP) of Yale, southern Burkina Faso, were conducted in 1994 and again in 1997 to assess the impacts of a tsetse control programme implemented from 1994, using insecticide-impregnated targets and pour-on treatments of all cattle with del...
Kamuanga, M.
,
Sigue, H.
,
Swallow, B.M.
,
Bauer, B.
,
D'Ieteren, G.D.M.
,
[Farmers' perceptions of the impacts of tsetse and trypanosomosis control on livestock production: Evidence from southern Burkina Faso]
,
Farmers' perceptions of the impacts of tsetse and trypanosomosis control on livestock production: Evidence from southern Burkina Faso
Twelve Boran steers with a mean live weight of 215.8 ± 13.9 kg were used in an incomplete Latin Square experiment to compare the apparent phosphorus (P) absorption in cattle when Napier grass (Pennisetum purpureum) was supplemented with Busumbu rock phosphate (BRP), Minjingu rock phosphate (MRP)...
Odongo, N.E.
,
Tanner, J.C.
,
Romney, D.L.
,
Plaizier, J.
,
Straaten, P. van
,
McBride, B.
,
[The effects of supplementing Napier grass (Pennisetum purpureum) with rock phosphate and steamed bone meal compared with a commercial mineral mix on phosphorus absorption in cattle]
,
The effects of supplementing Napier grass (Pennisetum purpureum) with rock phosphate and steamed bone meal compared with a commercial mineral mix on phosphorus absorption in cattle
There is growing evidence that immunity of cattle to Cowdria ruminantium infection is mediated by T lymphocytes. C. ruminantium antigens that stimulate these responses are therefore of considerable importance to the development of a sub-unit vaccine against the disease. We have examined T cell re...
Mwangi, D.M.
,
McKeever, Declan J.
,
Nyanjui, J.K.
,
Barbet, A.F.
,
Mahan, S.M.
,
[Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent]
,
Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent
The membrane-associated form of the variable surface glycoprotein (mfVSG) from African trypanosomes is a potent macrophage activator capable of inducing production of tumor necrosis factor z (TNFalfa) in both bovine and murine models. Using a bovine model. we have re-investigated the hypothesis t...
Sileghem, M.R.
,
Saya, R.
,
Grab, D.J.
,
Naessens, Jan
,
[An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes]
,
An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes
Major histocompatibility complex (MHC) class I restricted cellular immune responses play an important role in immunity to intracellular pathogens. By binding antigenic peptides and presenting them to T cells, class I molecules impose significant selection on the targets of immune responses. Candi...
Russell, G.C.
,
Oliver, R.A.
,
Craigmile, S.
,
Nene, Vishvanath
,
Glass, E.J.
,
[Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice]
,
Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice
Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...
Graham, S.P.
,
Saya, R.
,
Awino, Elias
,
Ngugi, D.
,
Nyanjui, J.K.
,
Hecker, R.
,
Taracha, E.L.N.
,
Nene, Vishvanath
,
[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]
,
Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva
Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (beta-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The...
Buza, J.
,
Naessens, Jan
,
[Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive]
,
Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive