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We developed an F(11) AIL population from an F(1) cross of A/J (susceptible) and C57BL/6J (resistant) mouse strains. One thousand F(11) mice were challenged with P.c. chabaudi 54X, and 340 mice selected from the phenotypic extremes for susceptibility and resistance were genotyped for microsatel...


Hernández Valladares, M.Naessens, JanGibson, John P.Musoke, A.J.Nagda, S.M.Rihet, P.Ole-MoiYoi, O.K.Iraqi, F.A.[Confirmation and dissection of QTL controlling resistance to malaria in mice]Confirmation and dissection of QTL controlling resistance to malaria in mice

DNA samples from 30 Friesian, 18 N'Dama, 30 Sahiwal and 18 Barotse (derived from Bos taurus and B. indicus) cattle were amplified by the polymerase chain reaction using previously described primer sequences for amplification of the Y chromosome locus INRA124. A polymorphism with 2 alleles was det...


Hanotte, Olivier H.Okomo, M.Verjee, Y.Rege, J.E.O.Teale, A.J.[A polymorphic Y chromosome microsatellite locus in cattle]A polymorphic Y chromosome microsatellite locus in cattle

The discipline of genomics is setting new paradigms in research approaches to resolving problems in human and animal health. We propose to determine the genome sequence of Theileria parva, a pathogen of cattle, using the random shotgun approach pioneered at The Institute for Genomic Research (TIG...


Nene, VishvanathBishop, Richard P.Morzaria, S.P.Gardner, M.J.Sugimoto, C.Ole-MoiYoi, O.K.Fraser, C.M.Irvin, A.[Theileria parva genomics reveals an atypical apicomplexan genome]Theileria parva genomics reveals an atypical apicomplexan genome
Liakat Ali, MSánchez, P.L.Yu, SLorieux, MEizenga, GC[Chromosome segment substitution lines: a powerful tool for the Introgression of valuable genes from oryza wild species into cultivated rice (O. sativa)]Chromosome segment substitution lines: a powerful tool for the Introgression of valuable genes from oryza wild species into cultivated rice (O. sativa)

The ploidy levels of the twenty-two yam (Dioscorea cayenensis-D. rotundata complex) cultivars within germplasm Cameroon Guinea yam were determined by flow cytometry. Three different ploidy levels (4x,6x, 8x) were detected within the samples analysed. Fifteen cultivars were tetraploids, five were ...


Dansi, A.Mignouna, H.Pillay, M.Zok, S.[Ploidy variation in the cultivated yams (Dioscorea cayenensisDioscorea rotundata complex) from Cameroon as determined by flow cytometry]Ploidy variation in the cultivated yams (Dioscorea cayenensisDioscorea rotundata complex) from Cameroon as determined by flow cytometry

Plantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of di...


Pillay, M.Nwakanma, D.Tenkouano, A.[Identification of RAPD markers linked to A and B genome sequences in Musa L]Identification of RAPD markers linked to A and B genome sequences in Musa L

Trypanosomiasis is one of the most important diseases in African cattle. About 3 million cattle die every year by this disease. However, about 5 percent of the total African cattle have a natural resistance against trypanosomiasis. In a preliminary study markers linked to trypanotolerance have be...


Goldammer, T.Brunner, R.M.Kang'a, S.Hanotte, Olivier H.Schwerin, M.[Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance]Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance

The genus Dioscorea is considered to be one of the most difficult groups for cyto-taxonomic studies because of the large number and small size of the chromosomes. The objective of the present study was to develop an efficient method of chromosome observation by identifying the optimum time for co...


Babil, P.K.Kikuno, H.Shiwachi, H.Toyohara, H.Fujigaki, J.Fujimaki, H.Asiedu, Robert[Optimum time for collection of root samples for chromosome observation in yams (Dioscorea spp.)]Optimum time for collection of root samples for chromosome observation in yams (Dioscorea spp.)

Cytological studies in Musa spp. L. are hampered by the small size and poor staining ability of the chromosomes. The aim of this experiment was to use silver nitrate as a staining agent for Musa chromosomes. The results of this study showed that silver nitrate is a useful alternative stain for cy...


Pillay, M.Adeleke, M.[Silver staining of Musa chromosomes]Silver staining of Musa chromosomes

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