Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...
Honda, Y.
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Matsubara, Y.
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Morzaria, S.P.
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McKeever, Declan J.
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[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]
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Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes
Theileria parva is an intracellular sporozoan parasite that infects and transforms bovine lymphocytes, causing a severe lymphoproliferative disease known as East Coast fever in eastern, central and southern Africa. In this article, Declan McKeever and colleagues summarize the current understandin...
McKeever, Declan J.
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Taracha, E.L.N.
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Morrison, W. Ivan
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Musoke, A.J.
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Morzaria, S.P.
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[Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies]
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Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies
Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...
Bishop, Richard P.
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Nene, Vishvanath
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Staeyert, J.
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Rowlands, G.J.
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Nyanjui, J.
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Osaso, J.
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Morzaria, S.P.
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Musoke, A.J.
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[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]
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Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...
Five different East Coast fever (ECF)-control strategies (involving ECF immunisation by the infection-and-treatment method) were tested in groups of traditionally managed Sanga cattle in the Central Province of Zambia over a period of 2.5 years. Two groups were under intensive tick control (weekl...
Minjauw, B.
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Rushton, Jonathan
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James, A.D.
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Upton, M.
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[Financial analysis of East Coast Fever control strategies in traditionally managed Sanga cattle in Central Province of Zambia]
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Financial analysis of East Coast Fever control strategies in traditionally managed Sanga cattle in Central Province of Zambia
East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
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Rowlands, G.J.
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Nene, Vishvanath
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Nyanjui, J.
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Katende, J.
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Spooner, P.R.
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Mwaura, S.
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Odongo, D.
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Nkonge, C.
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Mbogo, S.
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Bishop, Richard P.
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Morzaria, S.P.
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[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
The effects of holding temperature, pH and medium on the infectivity of Theileria parva sporozoites were investigated using an in vitro infectivity assay. The sporozoite infectivity lasted for 72 h at a holding temperature of 4 C but for only 24 h at 24 degrees C. Sporozoite infectivity was fo...
Kimbita, E.N.
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Silayo, R.S.
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Dolan, T.T.
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[Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity]
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Theileria parva: In vitro studies on the effects of holding temperature, PH and medium on sporozoite infectivity
Mumba, C.
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Skjerve, E.
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Rich, M.
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Rich, Karl M.
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[Application of system dynamics and participatory spatial group model building in animal health: A case study of East Coast Fever interventions in Lundazi and Monze districts of Zambia]
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Application of system dynamics and participatory spatial group model building in animal health: A case study of East Coast Fever interventions in Lundazi and Monze districts of Zambia