The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...
Lejon, Veerle
,
Claes, F.
,
Verloo, D.
,
Maina, M.
,
Urakawa, T.
,
Majiwa, P.A.O.
,
Buscher, P.
,
[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]
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Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels
Bovine babesiosis has been identified clinically in many areas of Morocco. A seroepidemiological survey of B. bigemina infection was conducted in the 5 main dairy cattle areas in order to estimate the prevalence of the infection. A total of 1819 bovine sera was tested using ELISA to identify ani...
El-Haj, N.
,
Kachani, M.
,
Ouhelli, H.
,
Bouslikhane, M.
,
Ahami, A.T.
,
El-Guennouni, R.
,
El-Hasnaoui, M.
,
Katende, J.M.
,
Morzaria, S.P.
,
[Etudes epidemiologiques sur Babesia bigemina au Maroc. (Epidemiological studies of Babesia bigemina infection in Morocco)]
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Etudes epidemiologiques sur Babesia bigemina au Maroc. (Epidemiological studies of Babesia bigemina infection in Morocco)
Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanosoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which ...
Davison, H.C.
,
Thrusfield, M.V.
,
Muharsini, S.
,
Husein, A.
,
Partoutomo, S.
,
Rae, P.F.
,
Masake, R.
,
Luckins, A.G.
,
[Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia]
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Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia
Taylor, K.A.
,
Gichuki, B.
,
Lutje, V.
,
Naessens, Jan
,
Williams, D.J.L.
,
[In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle]
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In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle
Describes experiments in which the effect of repeated freezing and thawing on the stability and activity of bovine antibodies has been studied using the enzyme linked immunosorbent assay (ELISA) against the infectious bovine rhinotrachietis/infections pustular vulvovaginitis (IBR/IPV); and using ...
Cecchini, G.
,
Tekelye Bekele
,
Kasali, O.B.
,
[The effect of repeated freezing and thawing of serum on the activity of antibodies]
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The effect of repeated freezing and thawing of serum on the activity of antibodies
Authié, E.
,
Duvallet, G.
,
Robertson, C.
,
Williams, D.J.L.
,
[Antibody response to a 33 kDa cysteine protease of Trypanosoma congolense: Relationship to 'trypanotolerance' in cattle]
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Antibody response to a 33 kDa cysteine protease of Trypanosoma congolense: Relationship to 'trypanotolerance' in cattle
Lutje, V.
,
Black, S.J.
,
[Analysis of pokeweed mitogen-induced in vitro proliferation and antibody responses of bovine lymphocytes]
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Analysis of pokeweed mitogen-induced in vitro proliferation and antibody responses of bovine lymphocytes
Katende, J.M.
,
Musoke, A.J.
,
Nantulya, V.M.
,
Goddeeris, B.M.
,
[A new method for fixation and preservation of trypanosomal antigens for use in the indirect immunofluorescent antibody test for diagnosis of bovine trypanosomiasis]
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A new method for fixation and preservation of trypanosomal antigens for use in the indirect immunofluorescent antibody test for diagnosis of bovine trypanosomiasis