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Assessment of the existing PCR-gel electrophoresis-based methods for detection of Rx1 and Rx2, the genes that independently control extreme resistance (ER) to Potato virus X (PVX), indicated that the 5Rx1F/5Rx1R primer pair led to reliable detection of Rx1 whereas the 106Rx2F/106Rx2R primer pair ...


Nie, X.Dickison, V.L.Brooks, S.Nie, B.Singh, M.Koeyer, D.L. deMurphy, A.M.[High resolution DNA melting assays for detection of Rx1 and Rx2 for high-throughput marker assisted selection for extreme resistance to Potato virus X in tetraploid potato]High resolution DNA melting assays for detection of Rx1 and Rx2 for high-throughput marker assisted selection for extreme resistance to Potato virus X in tetraploid potato

It is claimed that, with the exception of Musa balbisiana, all banana varieties are susceptible to bacterial wilt caused by Xanthomonas campestris pv. musacearum (Xcm). Despite being resistant to Xcm infection, M. balbisiana is not preferred for breeding because it belongs to the BB genome subgro...


Nakato, G.V.Christelova, P.Were, E.Nyine, M.Coutinho, T.A.Doležel, JaroslavUwimana, B.Swennen, R.Mahuku, George S.[Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt]Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt...


Wagaba, HenryPatil, Basavaprabhu L.Mukasa, SettumbaAlicai, TitusFauquet, Claude M.Taylor, Nigel J.[Artificial microRNA-derived resistance to Cassava brown streak disease]Artificial microRNA-derived resistance to Cassava brown streak disease

Four different types of arcelin, a protein that plays an important role in resistance to bruchids such as Zabrotes subfasciatus, were identified in wild accessions of Phaseolus vulgaris. Isogenic lines with cultivated black- (Porrillo 70) and white- (Sanilac) seeded parents were obtained by norma...


Harmsen, R.Bliss, Fredrick A.Cardona, C.POSSO, C.E.Osborn, Thomas C.[Transferring genes for arcelin protein from wild to cultivated beans: Implications for bruchid resistance]Transferring genes for arcelin protein from wild to cultivated beans: Implications for bruchid resistance

Fifty-three bean (Phaseolus vulgaris) accessions were evaluated for their reactions to infection by Macrophomina phaseolina under field and greenhouse conditions. A field nursery was established on an experimental farm near Santander de Quilichao, Colombia. All accessions were evaluated in paired...


Pastor Corrales, Marcial A.Abawi, G.S.[Reactions of selected bean accessions to infection by Macrophomina phaseolina]Reactions of selected bean accessions to infection by Macrophomina phaseolina

A Brazilian isolate of Fusarium oxysporum f. sp. phaseoli (F. o. f. sp. phaseoli) was used to determine the reactions of 66 lines of beans to the Fusarium yellows pathogen in greenhouse tests. The distal 1 cm of root tips of 1-wk-old seedlings grown in sterilized sand were removed, roots were dip...


Pastor Corrales, Marcial A.Abawi, G.S.[Reactions of selected bean germ plasm to infection by Fusarium oxysporum f. sp. phaseoli]Reactions of selected bean germ plasm to infection by Fusarium oxysporum f. sp. phaseoli

Over 150 bean (Phaseolus vulgaris) pure lines and accessions were evaluated in growth chamber and greenhouse trials for reactions to one or more populations of root-knot nematodes (Meloidogyne spp.) collected from Colombia or the United States. Bean pure lines and accessions were evaluated for ro...


Mullin, BAAbawi, G.S.Pastor Corrales, Marcial A.Kornegay, Julia L.[Reactions of selected bean pure lines and accessions to Meloidogyne species]Reactions of selected bean pure lines and accessions to Meloidogyne species

One necrosis-inducing strain and four mosaic-inducing strains of bean common mosaic virus (BCMV) were differentially seed-transmitted in 14 mosaic-susceptible bean (Phaseolus vulgaris) cultivars. Dubbele Witte, Redlands Greenleaf B, Michelite, Sanilac, and Red Mexican 35 consistently transmitted ...


Morales, Francisco JoséCastaño, M.[Seed transmission characteristics of selected bean common mosaic virus strains in differential bean cultivars]Seed transmission characteristics of selected bean common mosaic virus strains in differential bean cultivars

Bean germplasm was evaluated at CIAT for resistance to BCMV strains NL-4, NL-3, and NL-5 in order to use them in the national breeding and selection programs in Africa, Europe, USA, and Chile. Ninety-eight lines were selected for an international Bean Common Mosaic/Black Root resistance nursery. ...


Davis, J.H.C.Morales, Francisco JoséCastaño, M.[Resistance to black root]Resistance to black root

The resistance of 9 bean cv. to isolates of Thanatephorus cucumeris collected from either leaves (Th-5) or roots (Rs-8) of infected plants was tested under controlled conditions in growth chambers (22-25 degrees Celsius, 80-100 percent RH and 12 h artificial light). Adequate concn. of inoculum of...


Castaño J., Mauricio[Evaluacion de germoplasma de frijol (Phaseolus vulgaris L.) para resistencia a mustia hilachosa]Evaluacion de germoplasma de frijol (Phaseolus vulgaris L.) para resistencia a mustia hilachosa

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